Semaphorin 4D/CD100 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-86705

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (92%), Rat (90%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Semaphorin 4D/CD100 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LLIGKKKPKSDFCDREQSLKETLVEPGSFSQQNGEHPKPALDTGYETEQDTITSKVPTDREDSQRIDDLSARDKPFDVKCELKFADSDADGD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit Semaphorin 4D/CD100 Antibody - BSA Free (NBP1-86705) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Semaphorin 4D/CD100 Antibody - BSA Free

Immunohistochemistry-Paraffin: Semaphorin 4D/CD100 Antibody [NBP1-86705]

Immunohistochemistry-Paraffin: Semaphorin 4D/CD100 Antibody [NBP1-86705]

Immunohistochemistry-Paraffin: Semaphorin 4D/CD100 Antibody [NBP1-86705] - Staining of human cervix shows moderate granular cytoplasmic positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: Semaphorin 4D/CD100 Antibody [NBP1-86705]

Immunohistochemistry-Paraffin: Semaphorin 4D/CD100 Antibody [NBP1-86705]

Immunohistochemistry-Paraffin: Semaphorin 4D/CD100 Antibody [NBP1-86705] - Staining of human lymph node shows moderate membranous and cytoplasmic positivity in germinal and non-germinal center cells.
Immunohistochemistry-Paraffin: Semaphorin 4D/CD100 Antibody [NBP1-86705]

Immunohistochemistry-Paraffin: Semaphorin 4D/CD100 Antibody [NBP1-86705]

Immunohistochemistry-Paraffin: Semaphorin 4D/CD100 Antibody [NBP1-86705] - Staining of human colon shows strong membranous and ctyoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: Semaphorin 4D/CD100 Antibody [NBP1-86705]

Immunohistochemistry-Paraffin: Semaphorin 4D/CD100 Antibody [NBP1-86705]

Immunohistochemistry-Paraffin: Semaphorin 4D/CD100 Antibody [NBP1-86705] - Staining of human cerebral cortex shows moderate membranous and ctyoplasmic positivity in neuropil.
Semaphorin 4D/CD100 Antibody - BSA Free Western Blot: Semaphorin 4D/CD100 Antibody - BSA Free [NBP1-86705]

Western Blot: Semaphorin 4D/CD100 Antibody - BSA Free [NBP1-86705]

Analysis in human cell line HDLM-2.

Applications for Semaphorin 4D/CD100 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Semaphorin 4D/CD100

Semaphorins are neuronal chemorepellants that direct pioneering neurons during nervous system development. CD100, a 150 kDa homodimer, is a novel semaphorin that induces B cells to aggregate and improves their viability in vitro. CD100 modifies CD40-CD40L B cell signaling by augmenting B cell aggregation and survival and down regulating CD23 expression. CD72 is a lymphocyte receptor for the class IV semaphorin CD100, which is involved in regulating B cell signaling. CD100 is expressed on resting and PHA stimulated T cells. The protein is weakly expressed on NK cells, EBV transformed B cells, monocytes and tumor/peripheral blood T cell lines, and at higher density on activated T cells and B cells.

Alternate Names

A8, BB18, CD100, COLL-4, M-sema-G, SEMA4D, SEMAJ

Gene Symbol

SEMA4D

Additional Semaphorin 4D/CD100 Products

Product Documents for Semaphorin 4D/CD100 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Semaphorin 4D/CD100 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Proteoglycans

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Protocols

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