Siglec-2/CD22 Antibody (hL22 (Epratuzumab)) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-75188

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Cynomolgus Monkey, Rhesus Macaque

Applications

Block/Neutralize, Flow Cytometry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Human IgG1 kappa Clone # hL22 (Epratuzumab)

Format

Azide and BSA Free
View all Siglec-2/CD22 Primary Antibodies »

Product Specifications

Immunogen

This antibody was prepared by the humanization of LL2 (EPB-2), a murine anti-CD22 IgG2a raised against Raji Burkitt lymphoma cells. Murine sequences comprise 5-10% of the molecule, with the remainder being human framework sequences, which greatly reduces the potential for immunogenicity (Traczewski, 2010).

Specificity

This antibody is specific for the 3rd Ig-like domain of human CD22 (epitope B), a cell surface glycoprotein present on mature B-cells and on many types of malignant B-cells.

Clonality

Monoclonal

Host

Human

Isotype

IgG1 kappa

Applications for Siglec-2/CD22 Antibody (hL22 (Epratuzumab)) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:10 - 1:1000

Immunoprecipitation

1:10 - 1:500
Application Notes
Epratuzumab binds to the third extracellular domain of CD22, inducing CD22 phosphorylation, resulting in negative modulation of BCR activation, and rapid CD22 internalization, leading to modulation of B-cell homing (Traczewski, 2010). Initial phase II and two terminated early phase III studies suggest that the use of this antibody to treat systemic lupus erythematosus is effective and well tolerated, but both phase III trials failed to meet primary clinical efficacy endpoints. Additionally, in vitro studies and clinical trials indicate that this antibody can be used in combination therapy with another inhibitor of B-cell activity, rituximab (anti-CD20), in the treatment of non-Hodgkin lymphoma (Traczewski, 2010).

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: Siglec-2/CD22

CD22 is a single-pass type 1 membrane glycoprotein found in the cytoplasm and cell membrane of B-lymphocytes. CD22 has at least two isoforms (alpha and beta) produced by alternate splicing. The protein exists predominantly as a monomer of the beta isoform (95 kDa). It is also found as a heterodimer of the beta and alpha isoforms.

Long Name

Sialic Acid Binding Ig-like Lectin 2

Alternate Names

BL-CAM, CD22, Siglec2

Gene Symbol

CD22

Additional Siglec-2/CD22 Products

Product Documents for Siglec-2/CD22 Antibody (hL22 (Epratuzumab)) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Siglec-2/CD22 Antibody (hL22 (Epratuzumab)) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for Siglec-2/CD22 Antibody (hL22 (Epratuzumab)) - Azide and BSA Free

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  • Q: I am interested in buying a CD22 antibody for fluorescence microscopy and FACS I red on your web page that you also offer to label your antibodies, if required, is that correct?

    A: We do have the ability to perform custom conjugations on certain antibodies, including some to the CD22 protein. If you send me the conjugate you are interested in I can provide you with a quote.

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