SMYD4 Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-93044
![Immunohistochemistry-Paraffin: SMYD4 Antibody - BSA Free [NBP2-93044]](https://resources.rndsystems.com/images/products/SMYD4-Antibody-Immunohistochemistry-Paraffin-NBP2-93044-img0004.jpg "Immunohistochemistry-Paraffin: SMYD4 Antibody - BSA Free [NBP2-93044]")
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human SMYD4 (NP_443160.2). MDLPVDEWKSYLLQKWASLPTSVQVTISTAETLRDIFLHSSSLLQPEDELFLKRLSKGYLVGKDSDAPLFYREEGNKKFQEKDYTGAAVLYSKGVSHSRPNTEDMSLCHANRSAALFHLGQYETCLKDINRAQTHGYPERLQPKIMLRKAECLVALGRLQEASQTISDLERNFTATPALADVLPQTLQRNLHRLKMKMQEKDSLTESFPAALAKTLEDAALREENEQLSNASSSIGLCVDPLKGRCLVATKDILPGELLV
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
89 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for SMYD4 Antibody - BSA Free
Immunohistochemistry-Paraffin: SMYD4 Antibody - BSA Free [NBP2-93044]
Immunohistochemistry-Paraffin: SMYD4 Antibody [NBP2-93044] - Paraffin-embedded rat spleen using SMYD4.![Immunohistochemistry-Paraffin: SMYD4 Antibody - BSA Free [NBP2-93044]](https://resources.rndsystems.com/images/products/SMYD4-Antibody-Immunohistochemistry-Paraffin-NBP2-93044-img0002.jpg "Immunohistochemistry-Paraffin: SMYD4 Antibody - BSA Free [NBP2-93044]")
Immunohistochemistry-Paraffin: SMYD4 Antibody - BSA Free [NBP2-93044]
Immunohistochemistry-Paraffin: SMYD4 Antibody [NBP2-93044] - Paraffin-embedded human colon carcinoma using SMYD4.![Immunohistochemistry-Paraffin: SMYD4 Antibody - BSA Free [NBP2-93044]](https://resources.rndsystems.com/images/products/SMYD4-Antibody-Immunohistochemistry-Paraffin-NBP2-93044-img0003.jpg "Immunohistochemistry-Paraffin: SMYD4 Antibody - BSA Free [NBP2-93044]")
Immunohistochemistry-Paraffin: SMYD4 Antibody - BSA Free [NBP2-93044]
Immunohistochemistry-Paraffin: SMYD4 Antibody [NBP2-93044] - Paraffin-embedded mouse lung using SMYD4.
Immunoprecipitation: SMYD4 Antibody - BSA Free [NBP2-93044] -
Immunoprecipitation: SMYD4 Antibody - BSA Free [NBP2-93044] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug SMYD4 Rabbit pAb. Western blot was performed from the immunoprecipitate using SMYD4 Rabbit pAb at a dilition of 1:1000.
Immunocytochemistry/ Immunofluorescence: SMYD4 Antibody - BSA Free [NBP2-93044] -
Immunocytochemistry/ Immunofluorescence: SMYD4 Antibody - BSA Free [NBP2-93044] - Immunofluorescence analysis of A-549 cells using SMYD4 Rabbit pAb. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Western Blot: SMYD4 Antibody - BSA Free [NBP2-93044] -
Western Blot: SMYD4 Antibody - BSA Free [NBP2-93044] - Western blot analysis of lysates from HeLa cells, using SMYD4 Rabbit pAb at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Applications for SMYD4 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50-1:100
Immunohistochemistry
1:50-1:200
Immunoprecipitation
1:500 - 1:1000
Western Blot
1:1000 - 1:5000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: SMYD4
Alternate Names
KIAA1936ZMYND21, SET and MYND domain containing 4, SET and MYND domain-containing protein 4
Gene Symbol
SMYD4
Additional SMYD4 Products
Product Documents for SMYD4 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for SMYD4 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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