SOX10 Antibody (CL4455) - BSA Free

Novus Biologicals | Catalog # NBP2-59050

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Rat (98%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL4455

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P56693, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for SOX10 Antibody (CL4455) - BSA Free

Immunohistochemistry: SOX10 Antibody (CL4455) [NBP2-59050]

Immunohistochemistry: SOX10 Antibody (CL4455) [NBP2-59050]

Immunohistochemistry: SOX10 Antibody (CL4455) [NBP2-59050] - Staining of mouse embryo E11 shows nuclear immunoreactivity in the developing trigeminal ganglion and ear placode.
Immunohistochemistry-Paraffin: SOX10 Antibody (CL4455) [NBP2-59050]

Immunohistochemistry-Paraffin: SOX10 Antibody (CL4455) [NBP2-59050]

Immunohistochemistry-Paraffin: SOX10 Antibody (CL4455) [NBP2-59050] - Staining of human cerebral cortex shows strong nuclear immunoreactivity in a subset of glial cells.
Immunohistochemistry-Paraffin: SOX10 Antibody (CL4455) [NBP2-59050]

Immunohistochemistry-Paraffin: SOX10 Antibody (CL4455) [NBP2-59050]

Immunohistochemistry-Paraffin: SOX10 Antibody (CL4455) [NBP2-59050] - Staining of human breast shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: SOX10 Antibody (CL4455) [NBP2-59050]

Immunohistochemistry-Paraffin: SOX10 Antibody (CL4455) [NBP2-59050]

Immunohistochemistry-Paraffin: SOX10 Antibody (CL4455) [NBP2-59050] - Staining of human cervix shows nuclear immunoreactivity in a subset of basal cells in squamous epithelium.
SOX10 Antibody (CL4455) - BSA Free

SOX10 Antibody (CL4455) [NBP2-59050] -

Staining of mouse embryo E11 shows strong positivity in the developing trigeminal ganglion.
Immunohistochemistry-Paraffin: SOX10 Antibody (CL4455) [NBP2-59050]

Immunohistochemistry-Paraffin: SOX10 Antibody (CL4455) [NBP2-59050]

Immunohistochemistry-Paraffin: SOX10 Antibody (CL4455) [NBP2-59050] - Staining of human pancreas shows absence of positivity as expected (negative control).

Applications for SOX10 Antibody (CL4455) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Paraffin

1:2500 - 1:5000
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Reviewed Applications

Read 1 review rated 3 using NBP2-59050 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SOX10

SOX10 is a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. This protein may act as a transcriptional activator after forming a protein complex with other proteins. It acts as a nucleocytoplasmic shuttle protein and is important for neural crest and peripheral nervous system development. Mutations in this gene are associated with Waardenburg-Shah and Waardenburg-Hirschsprung disease.

Long Name

SRY-box transcription factor 10

Alternate Names

DOM, PCWH, SOX-10, WS2E, WS4, WS4C

Gene Symbol

SOX10

Additional SOX10 Products

Product Documents for SOX10 Antibody (CL4455) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for SOX10 Antibody (CL4455) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for SOX10 Antibody (CL4455) - BSA Free (1)

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Customer Images

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  • SOX10 Antibody (CL4455)
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: Adult spinal cord
    Species: Feline
    Verified Customer | Posted 03/14/2019
    Image was captured using fluorescent microscopy demonstrating SOX10 (red) and DAPI (blue).
    For NBP2-59050 antibody, I used it at 1:200 in 1X PBS + 0.05% tween 20+ 2% BSA. As I tested it at 1:50, 1:100, 1:200, 1:500 and 1:1000, that concentration gave the best signal/noise ratio for IHC-Fr. The slides were subsequently incubated with alexa fluor conjugated secondary antibody for 1hr at RT and imaged with fluorescent microscopy.
    SOX10 Antibody (CL4455) - BSA Free NBP2-59050

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