![Immunocytochemistry/ Immunofluorescence: SP4 Antibody [NBP2-31983]](https://resources.rndsystems.com/images/products/SP4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-31983-img0006.jpg "Immunocytochemistry/ Immunofluorescence: SP4 Antibody [NBP2-31983]")
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Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: NQATGQQQIIIDPSQGLVQLQNQPQQLELVTTQLAGNAWQLVASTPPASKENNVSQP
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SP4 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence: SP4 Antibody [NBP2-31983]
Immunocytochemistry/Immunofluorescence: SP4 Antibody [NBP2-31983] - Staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.![Immunohistochemistry-Paraffin: SP4 Antibody [NBP2-31983]](https://resources.rndsystems.com/images/products/SP4-Antibody-Immunohistochemistry-NBP2-31983-img0002.jpg "Immunohistochemistry-Paraffin: SP4 Antibody [NBP2-31983]")
Immunohistochemistry-Paraffin: SP4 Antibody [NBP2-31983]
Immunohistochemistry-Paraffin: SP4 Antibody [NBP2-31983] - Staining of human lymph node shows distinct nuclear positivity in non-germinal center cells.![SP4 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: SP4 Antibody - BSA Free [NBP2-31983]](https://resources.rndsystems.com/images/products/nbp2-31983_rabbit-polyclonal-sp4-antibody-25620259121929.jpg "Chromatin Immunoprecipitation-exo-Seq: SP4 Antibody - BSA Free [NBP2-31983]")
Chromatin Immunoprecipitation-exo-Seq: SP4 Antibody - BSA Free [NBP2-31983]
ChIP-Exo-Seq composite graph for Anti-SP4 (NBP2-31983) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.
SP4 Antibody [NBP2-31983] -
Staining of human tonsil tissue shows moderate nuclear positivity in lymphoid cells.
SP4 Antibody [NBP2-31983] -
Staining of human kidney shows moderate nuclear positivity in cells in glomeruli with additional cytoplasmic positivity.
SP4 Antibody [NBP2-31983] -
Staining of human skin shows strong membranous-cytoplasmic positivity in squamous epithelial cells.
SP4 Antibody [NBP2-31983] -
Staining of human rectum shows moderate nuclear positivity in lymphoid cells with additional cytoplasmic positivity in glandular cells.Applications for SP4 Antibody - BSA Free
Application
Recommended Usage
Chromatin Immunoprecipitation
1-10µg per reaction
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SP4
Alternate Names
MGC130008, MGC130009, Sp4 transcription factor, SPR-1HF1B, transcription factor Sp4
Gene Symbol
SP4
UniProt
Additional SP4 Products
Product Documents for SP4 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for SP4 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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