SRCAP Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-84063

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Chromatin Immunoprecipitation-exo-Seq

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: AQALASPESLELASVASSETSSLSLVPPKDLLPVAVEILPVSEKNLSLTPSAPSLTLEAGSIPNGQEQEAPDSAEGTTLTVLPEGEELPLCVSESNGLELPPSAASDEPLQEPLEADRTSEELTEAKTPTSS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SRCAP Antibody - BSA Free

Immunohistochemistry-Paraffin: SRCAP Antibody [NBP1-84063]

Immunohistochemistry-Paraffin: SRCAP Antibody [NBP1-84063]

Immunohistochemistry-Paraffin: SRCAP Antibody [NBP1-84063] - Staining of human Testis shows moderate nuclear positivity in cells in seminiferous ducts and leydig cells.
Immunohistochemistry-Paraffin: SRCAP Antibody [NBP1-84063]

Immunohistochemistry-Paraffin: SRCAP Antibody [NBP1-84063]

Immunohistochemistry-Paraffin: SRCAP Antibody [NBP1-84063] - Staining of human Cerebellum shows moderate nuclear positivity in Purkinje cells.
Immunohistochemistry-Paraffin: SRCAP Antibody [NBP1-84063]

Immunohistochemistry-Paraffin: SRCAP Antibody [NBP1-84063]

Immunohistochemistry-Paraffin: SRCAP Antibody [NBP1-84063] - Staining of human Liver shows very weak nuclear positivity in hepatocytes.
Immunohistochemistry-Paraffin: SRCAP Antibody [NBP1-84063]

Immunohistochemistry-Paraffin: SRCAP Antibody [NBP1-84063]

Immunohistochemistry-Paraffin: SRCAP Antibody [NBP1-84063] - Staining of human Skin shows moderate nuclear positivity in squamous epithelial cells.
SRCAP Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: SRCAP Antibody - BSA Free [NBP1-84063]

Chromatin Immunoprecipitation-exo-Seq: SRCAP Antibody - BSA Free [NBP1-84063]

ChIP-Exo-Seq composite graph for Anti-SRCAP (NBP1-84063) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for SRCAP Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SRCAP

Catalytic component of the SRCAP complex which mediates the ATP-dependent exchange of histone H2AZ/H2B dimers for nucleosomal H2A/H2B, leading to transcriptional regulation of selected genes by chromatin remodeling. Acts as a coactivator for CREB-mediated transcription, steroid receptor-mediated transcription, and Notch-mediated transcription

Alternate Names

domino homolog 1, Domino homolog 2, DOMO1, EAF1, EC 3.6.1, EC 3.6.4.-, FLJ44499, KIAA0309helicase SRCAP, Snf2-related CBP activator, Snf2-related CREBBP activator protein, Swi2/Snf2-related ATPase homolog, domino homolog 1, SWR1Snf2-related CBP activator protein

Gene Symbol

SRCAP

Additional SRCAP Products

Product Documents for SRCAP Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for SRCAP Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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