ST2/IL-33R Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-85251
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Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: PDVLENKCGYTLCIYGRDMLPGEDVVTAVETNIRKSRRHIFILTPQITHNKEFAYEQEVALHCALIQNDAKVILIEMEALSELDMLQAEALQDSLQHLMKVQGTIKWREDHIANKRSLNSKFWKHVRYQMPVPSKIPRKASSLTPL
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit ST2/IL-33R Antibody - BSA Free (NBP1-85251) is a polyclonal antibody validated for use in IHC and WB. Anti-ST2/IL-33R Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for ST2/IL-33R Antibody - BSA Free
Western Blot: ST2/IL-33R Antibody [NBP1-85251]
Western Blot: ST2/IL-33 R Antibody [NBP1-85251] - Analysis in human cell line HEL.Immunohistochemistry-Paraffin: ST2/IL-33R Antibody [NBP1-85251]
Immunohistochemistry-Paraffin: ST2/IL-33 R Antibody [NBP1-85251] - Staining of human placenta shows moderate membranous positivity in trophoblastic cells.Immunohistochemistry-Paraffin: ST2/IL-33R Antibody [NBP1-85251]
Immunohistochemistry-Paraffin: ST2/IL-33 R Antibody [NBP1-85251] - Staining of human stomach shows moderate membranous positivity in glandular cells.Immunohistochemistry-Paraffin: ST2/IL-33R Antibody [NBP1-85251]
Immunohistochemistry-Paraffin: ST2/IL-33 R Antibody [NBP1-85251] - Staining of human kidney shows moderate membranous positivity in cells in glomeruli.Immunohistochemistry-Paraffin: ST2/IL-33R Antibody [NBP1-85251]
Immunohistochemistry-Paraffin: ST2/IL-33 R Antibody [NBP1-85251] - Staining of human lung shows moderate membranous positivity in pneumocytes.Applications for ST2/IL-33R Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500 - 1:1000
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Reviewed Applications
Read 1 review rated 5 using NBP1-85251 in the following applications:
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ST2/IL-33R
Long Name
Interleukin 33 Receptor
Alternate Names
DER4, Fit-1, IL-1 R4, IL-1R4, IL-1RL1, IL-33 R, IL-33R, IL1R4, IL33R, Ly84, ST2L, ST2V, T1
Gene Symbol
IL1RL1
Additional ST2/IL-33R Products
Product Documents for ST2/IL-33R Antibody - BSA Free
Product Specific Notices for ST2/IL-33R Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for ST2/IL-33R Antibody - BSA Free
Customer Reviews for ST2/IL-33R Antibody - BSA Free (1)
5 out of 5
1 Customer Rating
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Application: Immunohistochemistry-ParaffinSample Tested: FFPESpecies: HumanVerified Customer | Posted 01/07/2015Antibody did not stain skeletal muscle, as described in the literature
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for ST2/IL-33R Antibody - BSA Free
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A: The immunogen sequence for IL1RL1 Antibody (NBP1-85251) is stated on its datasheet. I have compared this for you with the ST2 isoforms as stated on UniProt. I can tell you that the immunogen matches perfectly with ST2L, and not with ST2S or ST2V. This is because the immunogen sequence corresponds to a region close to the C terminus of ST2L, within the sections missing in ST2S and ST2V (according to UniProt).
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