SUB1 Antibody

Novus Biologicals | Catalog # NB100-59775

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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Predicted:

Chicken (100%), Primate (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all SUB1 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 77 and 127 of human positive cofactor 4 using the numbering given in entry NP_006704.2 (GeneID 10923).

Reactivity Notes

Orangutan (100%). Rat reactivity reported from a verified customer review.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SUB1 Antibody

Western Blot: SUB1 Antibody [NB100-59775]

Western Blot: SUB1 Antibody [NB100-59775]

Western Blot: SUB1 Antibody [NB100-59775] - Analysis using rat cardiomyocyte lysate with anti-SUB1 antibody. Image from verified customer review.
Immunohistochemistry-Paraffin: SUB1 Antibody [NB100-59775]

Immunohistochemistry-Paraffin: SUB1 Antibody [NB100-59775]

Immunohistochemistry-Paraffin: SUB1 Antibody [NB100-59775] - Sample: FFPE section of human prostate carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti- PC4 used at a dilution of 1:200 (1ug/ml) and 1:1,000 (0.2ug/ml). Detection: DAB
Immunoprecipitation: SUB1 Antibody [NB100-59775]

Immunoprecipitation: SUB1 Antibody [NB100-59775]

Immunoprecipitation: SUB1 Antibody [NB100-59775] - Detection of human SUB1/PC4 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SUB1 antibody NB100-59775 used for WB at 0.04 ug/ml (A) and 0.1 ug/ml (B). SUB1 was immunoprecipitated by rabbit anti-SUB1 antibody NB100-59774, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).

Applications for SUB1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:2000

Immunohistochemistry-Paraffin

1:100-1:500

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Reviewed Applications

Read 1 review rated 5 using NB100-59775 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SUB1

PC4 is a multifunctional transcriptional coactivator that plays an important role in transcription, repair, and replication. PC4 interacts with general transcription factors, DNA repair factors, and mRNA processing factors to influence transcription initiation, coactivation, polyadenylation, transcription termination, and transcriptional repression. PC4 has been demonstrated to be a DNA binding protein and a bona fide component of chromatin with chromatin-compacting ability.

Alternate Names

activated RNA polymerase II transcription cofactor 4, activated RNA polymerase II transcriptional coactivator p15, MGC102747, p15, PC4p14P15, Positive cofactor 4, RPO2TC1, SUB1 homolog, SUB1 homolog (S. cerevisiae)

Entrez Gene IDs

10923 (Human)

Gene Symbol

SUB1

UniProt

NP_006704.2 (Human)

Additional SUB1 Products

Product Documents for SUB1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for SUB1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for SUB1 Antibody (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

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  • SUB1 Antibody
    Name: Shinichi Oka
    Application: Western Blot
    Sample Tested: primary neonatal ventricular cardiomyocytes
    Species: Rat
    Verified Customer | Posted 09/08/2022
    Western blot using rat cardiomyocyte lysate with anti-SUB1 antibody.
    SUB1 Antibody NB100-59775
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

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Protocols

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FAQs

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