SUPT16H Antibody (OTI3B8)

Novus Biologicals | Catalog # NBP3-44820

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3B8
View all SUPT16H Primary Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 187-282 of human SUPT16H (NP_009123) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for SUPT16H Antibody (OTI3B8)

SUPT16H Antibody (OTI3B8) Western Blot;SUPT16H Antibody (OTI3B8)[NBP3-44820]-

Western Blot;SUPT16H Antibody (OTI3B8)[NBP3-44820]-

Western blot analysis of extracts (35ug) from 3 different cell lines and human kidney tissue lysate by using anti-SUPT16H monoclonal antibody (1:500).
SUPT16H Antibody (OTI3B8) Immunohistochemistry;SUPT16H Antibody (OTI3B8)[NBP3-44820]-

Immunohistochemistry;SUPT16H Antibody (OTI3B8)[NBP3-44820]-

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SUPT16H mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.
SUPT16H Antibody (OTI3B8) Western Blot;SUPT16H Antibody (OTI3B8)[NBP3-44820]-

Western Blot;SUPT16H Antibody (OTI3B8)[NBP3-44820]-

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SUPT16H (, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SUPT16H (1:2000).

Applications for SUPT16H Antibody (OTI3B8)

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

>95%

Reconstitution

For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized powder ( 1X PBS, pH 7.3,)

Preservative

8% Trehalose

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 degrees C. Avoid freeze/thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SUPT16H

Transcription of protein-coding genes can be reconstituted on naked DNA with only the general transcription factors and RNA polymerase II. However, this minimal system cannot transcribe DNA packaged into chromatin, indicating that accessory factors may facilitate access to DNA. One such factor, FACT (facilitates chromatin transcription), interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT is composed of an 80 kDa subunit and a 140 kDa subunit; this gene encodes the 140 kDa subunit. (provided by RefSeq)

Alternate Names

CDC68, Chromatin-specific transcription elongation factor 140 kDa subunit, facilitates chromatin remodeling 140 kDa subunit, Facilitates chromatin transcription complex subunit SPT16, FACT 140 kDa subunit, FACT complex subunit SPT16, FACT140, FACTp140, FACTP140FACT, FLJ10857, FLJ14010, FLJ34357, hSPT16, SPT16/CDC68, suppressor of Ty (S.cerevisiae) 16 homolog, suppressor of Ty 16 homolog (S. cerevisiae)

Gene Symbol

SUPT16H

Additional SUPT16H Products

Product Documents for SUPT16H Antibody (OTI3B8)

Certificate of Analysis

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Product Specific Notices for SUPT16H Antibody (OTI3B8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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