Tau Antibody

Tau in Human Alzheimer's Disease Brain.

Tau was detected in immersion fixed paraffin-embedded sections of human Alzheimer's disease brain (cortex) using 5 µg/mL Goat Anti-Tau Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3494) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Tau by Immunocytochemistry/ Immunofluorescence

Progressive Plaque Pathology without Tau Pathology in APP/PS1+Tau Mice(A) APP/PS1+Tau mouse model was generated by breeding two feeder lines to produce four experimental genotypes of F1 littermates on a consistent outbred strain background.(B) Behavior, pathology, and recovery with tau transgene suppression were characterized over time.(C and D) Staining with thioflavin S (C) shows progressive plaque accumulation in APP/PS1+Tau and APP/PS1 mice. APP/PS1+Tau mice have significantly lower cortical plaque burden than APP/PS1 mice (D, two-way ANOVA, effect of genotype F[2,26] = 8.454, p = 0.007).(E) Tau is present in 14.5-month-old APP/PS1+Tau mice as shown with a total tau stain, but tau pathology does not accumulate in cell bodies or in dystrophic neurites around plaques, as shown by staining with phospho-tau (PHF1 and AT8) or misfolded tau (Alz50) antibodies, which all label tangle pathology in rTg4510-positive control sections.(F) None of the genotypes experienced age-related cortical atrophy (two-way ANOVA, effect of age, p > 0.05).Data shown are means ± SE. Dots on bar graphs represent means of individual animals (n per group, biological replicates, shown in each bar). Scale bars represent 1 mm (C, insets 100 × 100 μm) and 30 μm (E). See also Figure S1 and Table S1. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31825838), licensed under a CC-BY license. Not internally tested by R&D Systems.