TBCC Antibody (OTI4C4)

Novus Biologicals | Catalog # NBP2-02037

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Monkey, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI4C4
View all TBCC Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human TBCC(NP_003183) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TBCC Antibody (OTI4C4)

Western Blot: TBCC Antibody (4C4) [NBP2-02037] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-TBCC monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunocytochemistry/ Immunofluorescence: TBCC Antibody (OTI4C4) [NBP2-02037]

Immunocytochemistry/ Immunofluorescence: TBCC Antibody (OTI4C4) [NBP2-02037]

Immunocytochemistry/Immunofluorescence: TBCC Antibody (4C4) [NBP2-02037] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY TBCC.
Immunohistochemistry-Paraffin: TBCC Antibody (OTI4C4) [NBP2-02037]

Immunohistochemistry-Paraffin: TBCC Antibody (OTI4C4) [NBP2-02037]

Immunohistochemistry-Paraffin: TBCC Antibody (4C4) [NBP2-02037] - Staining of paraffin-embedded Human prostate tissue using anti-TBCC mouse monoclonal antibody.
Western Blot: TBCC Antibody (OTI4C4) [NBP2-02037]

Western Blot: TBCC Antibody (OTI4C4) [NBP2-02037]

Western Blot: TBCC Antibody (4C4) [NBP2-02037] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TBCC (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TBCC.
Immunohistochemistry-Paraffin: TBCC Antibody (OTI4C4) [NBP2-02037]

Immunohistochemistry-Paraffin: TBCC Antibody (OTI4C4) [NBP2-02037]

Immunohistochemistry-Paraffin: TBCC Antibody (4C4) [NBP2-02037] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-TBCC mouse monoclonal antibody.
Immunohistochemistry-Paraffin: TBCC Antibody (OTI4C4) [NBP2-02037]

Immunohistochemistry-Paraffin: TBCC Antibody (OTI4C4) [NBP2-02037]

Immunohistochemistry-Paraffin: TBCC Antibody (4C4) [NBP2-02037] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TBCC mouse monoclonal antibody.
Immunohistochemistry-Paraffin: TBCC Antibody (OTI4C4) [NBP2-02037]

Immunohistochemistry-Paraffin: TBCC Antibody (OTI4C4) [NBP2-02037]

Immunohistochemistry-Paraffin: TBCC Antibody (4C4) [NBP2-02037] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TBCC mouse monoclonal antibody.
Immunohistochemistry-Paraffin: TBCC Antibody (OTI4C4) [NBP2-02037]

Immunohistochemistry-Paraffin: TBCC Antibody (OTI4C4) [NBP2-02037]

Immunohistochemistry-Paraffin: TBCC Antibody (4C4) [NBP2-02037] - Staining of paraffin-embedded Human colon tissue using anti-TBCC mouse monoclonal antibody.
Immunohistochemistry-Paraffin: TBCC Antibody (OTI4C4) [NBP2-02037]

Immunohistochemistry-Paraffin: TBCC Antibody (OTI4C4) [NBP2-02037]

Immunohistochemistry-Paraffin: TBCC Antibody (4C4) [NBP2-02037] - Staining of paraffin-embedded Human pancreas tissue using anti-TBCC mouse monoclonal antibody.

Applications for TBCC Antibody (OTI4C4)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TBCC

Cofactor C is one of four proteins (cofactors A, D, E, and C) involved in the pathway leading to correctly folded beta-tubulin from folding intermediates. Cofactors A and D are believed to play a role in capturing and stabilizing beta-tubulin intermediates in a quasi-native confirmation. Cofactor E binds to the cofactor D/beta-tubulin complex; interaction with cofactor C then causes the release of beta-tubulin polypeptides that are committed to the native state. [provided by RefSeq].

Alternate Names

CFCtubulin-specific chaperone c, tubulin folding cofactor C, Tubulin-folding cofactor C

Entrez Gene IDs

6903 (Human)

Gene Symbol

TBCC

Additional TBCC Products

Product Documents for TBCC Antibody (OTI4C4)

Certificate of Analysis

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Product Specific Notices for TBCC Antibody (OTI4C4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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