TERF2IP Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-82433

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all TERF2IP Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: REFEEVVVDESPPDFEIHITMCDDDPPTPEEDSETQPDEEEEEEEEKVSQPEVGAAIKIIRQLMEKFNLDLSTVTQAFLKNSGELEATSAFLASGQRADGYPIWSRQDDIDLQKDDEDTREALVKKFGAQNVAR

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (84%), Rat (83%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TERF2IP Antibody - BSA Free

Western Blot: TERF2IP Antibody [NBP1-82433]

Western Blot: TERF2IP Antibody [NBP1-82433]

Western Blot: TERF2IP Antibody [NBP1-82433] - Analysis in control (vector only transfected HEK293T lysate) and TERF2IP over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: TERF2IP Antibody [NBP1-82433]

Immunocytochemistry/ Immunofluorescence: TERF2IP Antibody [NBP1-82433]

Immunocytochemistry/Immunofluorescence: TERF2IP Antibody [NBP1-82433] - Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear bodies.
Immunohistochemistry-Paraffin: TERF2IP Antibody [NBP1-82433]

Immunohistochemistry-Paraffin: TERF2IP Antibody [NBP1-82433]

Immunohistochemistry-Paraffin: TERF2IP Antibody [NBP1-82433] - Immunohistochemical staining of human lymph node shows strong nuclear positivity in reaction center cells.
Western Blot: TERF2IP Antibody [NBP1-82433]

Western Blot: TERF2IP Antibody [NBP1-82433]

Western Blot: TERF2IP Antibody [NBP1-82433] - Analysis in human cell line SCLC-21H.
TERF2IP Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: TERF2IP Antibody - BSA Free [NBP1-82433]

Chromatin Immunoprecipitation-exo-Seq: TERF2IP Antibody - BSA Free [NBP1-82433]

ChIP-Exo-Seq composite graph for Anti-TERF2IP (NBP1-82433) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for TERF2IP Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TERF2IP

Mammalian telomeric proteins, including TRF1, TRF2, tankyrase, and TIN2 have no recognized orthologs in budding yeast. However, human Rap1 (hRap1) is an ortholog of the yeast telomeric protein, scRap1p. hRap1 is a homodimer, has three conserved sequence motifs in common with scRap1, is located at telomeres, and plays a role in telomere length regulation. Although scRap1 binds telomeric DNA directly, hRap1 is recruited to telomeres by TRF2 and binds it with its C-terminus, rather than directly binding DNA.

Alternate Names

dopamine receptor interacting protein 5, Dopamine receptor-interacting protein 5, DRIP5, hRap1, RAP1 homolog, RAP1TERF2-interacting telomeric protein 1, Repressor/activator protein 1 homolog, telomeric repeat binding factor 2, interacting protein, telomeric repeat-binding factor 2-interacting protein 1, TRF2-interacting telomeric protein 1, TRF2-interacting telomeric RAP1 protein

Gene Symbol

TERF2IP

Additional TERF2IP Products

Product Documents for TERF2IP Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for TERF2IP Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TERF2IP Antibody - BSA Free

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Protocols

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