Thrombopoietin R/Tpo R Antibody (JE58-00)

Western Blot: Thrombopoietin R/Tpo R Antibody (JE58-00) [NBP3-33039]

Western Blot: Thrombopoietin R/Tpo R Antibody (JE58-00) [NBP3-33039] - Western blot analysis of Thrombopoietin R/Tpo R on different lysates with Rabbit anti-Thrombopoietin R/Tpo R antibody (NBP3-33039) at 1/1,000 dilution.

Lane 1: Mouse placenta tissue lysate (40 ug/Lane)
Lane 2: NIH/3T3 cell lysate (20 ug/Lane)
Lane 3: A431 cell lysate (no heat) (20 ug/Lane)
Lane 4: Rat spleen tissue lysate (40 ug/Lane)
Lane 5: Mouse spleen tissue lysate (40 ug/Lane)

Predicted band size: 71 kDa
Observed band size: 80 kDa

Exposure time: Lane 1-3: 24 seconds; Lane 4-5: 30 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33039) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Flow Cytometry: Thrombopoietin R/Tpo R Antibody (JE58-00) [NBP3-33039]

Flow Cytometry: Thrombopoietin R/Tpo R Antibody (JE58-00) [NBP3-33039] - Flow cytometric analysis of A431 cells labeling Thrombopoietin R/Tpo R.

Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33039, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).