TIM-4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87569

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Multiplex Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all TIM-4 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VFTTANTCLSLTPSTLPEEATGLLTPEPSKEGPILTAESETVLPSDSWSSVESTSADTVLLTSKESKVWDLPSTSHVSMWKTSDSVSSPQPGASDTAVPEQNKTTKTGQMDGIPMSMKNEMP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit TIM-4 Antibody - BSA Free (NBP1-87569) is a polyclonal antibody validated for use in Multiplex Immunofluorescence, IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for TIM-4 Antibody - BSA Free

Western Blot: TIM-4 Antibody [NBP1-87569]

Western Blot: TIM-4 Antibody [NBP1-87569]

Western Blot: TIM-4 Antibody [NBP1-87569] - Analysis in control (vector only transfected HEK293T lysate) and TIMD4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: TIM-4 Antibody [NBP1-87569]

Immunohistochemistry-Paraffin: TIM-4 Antibody [NBP1-87569]

Immunohistochemistry-Paraffin: TIM-4 Antibody [NBP1-87569] - Staining of human lymph node shows distinct positivity in macrophages.

Applications for TIM-4 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500-1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TIM-4

The T cell immunoglobulin and mucin domain containing protein (TIM) family encodes cell surface receptors that are involved in the regulation of T helper (Th) -1 and -2 cell-mediated immunity. Studies have shown that TIM 4, which is preferentially expressed on macrophages and dendritic cells, is the natural ligand of TIM 1, and that this binding leads to T-cell expansion and cytokine production. Unlike other members of the TIM family, TIM 4 lacks a putative tyrosine phosphorylation signal sequence in its intracellular domain (Kuchroo et al, 2003). The TIM 4 gene maps to a locus associated with predisposition to asthma in both mice and humans and with its connection to TIM 1-triggered Th2 responsiveness, may be considered as a candidate disease/predisposition gene for asthma

Long Name

T Cell Immunoglobulin Mucin-4

Alternate Names

Smuckler, TIM4, TIMD4

Gene Symbol

TIMD4

Additional TIM-4 Products

Product Documents for TIM-4 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for TIM-4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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