TIP30 Antibody

Novus Biologicals | Catalog # NBP2-20646

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (94%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all TIP30 Primary Antibodies »

Product Specifications

Immunogen

Full length human TIP30 Recombinant protein.

Reactivity Notes

Rat (85%).

Localization

Cytoplasm; Nucleus envelope

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TIP30 Antibody

Western Blot: TIP30 Antibody [NBP2-20646]

Western Blot: TIP30 Antibody [NBP2-20646]

Western Blot: TIP30 Antibody [NBP2-20646] - Sample (30 ug of whole cell lysate) A: A431 B: HeLa 12% SDS PAGE gel, diluted at 1:5000.
Immunocytochemistry/ Immunofluorescence: TIP30 Antibody [NBP2-20646]

Immunocytochemistry/ Immunofluorescence: TIP30 Antibody [NBP2-20646]

Immunocytochemistry/Immunofluorescence: TIP30 Antibody [NBP2-20646] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using TIP30 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.
Immunohistochemistry-Paraffin: TIP30 Antibody [NBP2-20646]

Immunohistochemistry-Paraffin: TIP30 Antibody [NBP2-20646]

Immunohistochemistry-Paraffin: TIP30 Antibody [NBP2-20646] - Paraffin-embedded gastric cancer. TIP30 antibody dilution: 1:500.
Immunohistochemistry-Paraffin: TIP30 Antibody [NBP2-20646]

Immunohistochemistry-Paraffin: TIP30 Antibody [NBP2-20646]

Immunohistochemistry-Paraffin: TIP30 Antibody [NBP2-20646] - Immunohistochemical analysis of paraffin-embedded Breast CA, using antibody at 1:250 dilution.
Immunohistochemistry-Paraffin: TIP30 Antibody [NBP2-20646]

Immunohistochemistry-Paraffin: TIP30 Antibody [NBP2-20646]

Immunohistochemistry-Paraffin: TIP30 Antibody [NBP2-20646] - Paraffin-embedded mouse muscle. TIP30 antibody diluted at 1:500.
TIP30 Antibody

Immunoprecipitation: TIP30 Antibody [NBP2-20646] -

TIP30 antibody immunoprecipitates TIP30 protein in IP experiments. IP Sample: hela whole cell lysate/extract A. 40 ug hela whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of TIP30 protein by 2 ug of TIP30 antibody (NBP2-20646) 10% SDS-PAGE The immunoprecipitated TIP30 protein was detected by TIP30 antibody (NBP2-20646) diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent

Applications for TIP30 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TIP30

Oxidoreductase required for tumor suppression. NAPDH-bound form inhibits nuclear import by competing with nuclear import substrates for binding to a subset of nuclear transport receptors. May act as a redox sensor linked to transcription through regulation of nuclear import. Isoform 1 is a metastasis suppressor with proapoptotic as well as antiangiogenic properties. Isoform 2 has an antiapoptotic effect.

Alternate Names

30 kDa HIV-1 TAT-interacting protein, CC3oxidoreductase HTATIP2, EC 1.1.1, EC 1.1.1.-, FLJ26963, HIV-1 Tat interactive protein 2, 30 kDa, HIV-1 Tat interactive protein 2, 30kDa, HIV-1 TAT-interactive protein 2, SDR44U1, Tat-interacting protein (30kD), TIP30short chain dehydrogenase/reductase family 44U, member 1

Gene Symbol

HTATIP2

UniProt

Q9BUP3 (Human)

Additional TIP30 Products

Product Documents for TIP30 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for TIP30 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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