TLE1 Antibody (MSVA-856R) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-57360

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # MSVA-856R

Format

Azide and BSA Free
View all TLE1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human TLE1fragment Swissprot Q04724

Localization

Nucleus

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody is highly specific and optimized for use in Immunohistochemistry.

It has been validated on TMAs covering 76 normal tissues and numerous cancer tissues.

Positive Control
Postive control:Tonsil: A weak to moderate TLE1 staining should be seen in suprabasal cells of squamous epithelium and in a small subset of lymphocytes in germinal centres.

Negative Control
Negitve control:Tonsil: The majority of lymphocytes in germinal centres should be TLE1 negative.

Scientific Data Images for TLE1 Antibody (MSVA-856R) - Azide and BSA Free

TLE1 Antibody (MSVA-856R) - Azide and BSA Free

Immunohistochemistry-Paraffin:TLE1 Antibody (MSVA-856R) - Azide and BSA FreeNBP3-57360

Synovial sarcoma with strong TLE1 positivity of all tumor cells
TLE1 Antibody (MSVA-856R) - Azide and BSA Free

Immunohistochemistry-Paraffin:TLE1 Antibody (MSVA-856R) - Azide and BSA FreeNBP3-57360

Lymph node with weak to moderate TLE1 staining in a subset of lymphocytes predominantly in germinal centres
TLE1 Antibody (MSVA-856R) - Azide and BSA Free

Immunohistochemistry-Paraffin:TLE1 Antibody (MSVA-856R) - Azide and BSA FreeNBP3-57360

Endometrium with moderate to strong TLE1 staining of epithelial and stromal cells

Applications for TLE1 Antibody (MSVA-856R) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TLE1

Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG

Long Name

Transducin-like Enhancer of Split 1

Alternate Names

GRG1

Gene Symbol

TLE1

Additional TLE1 Products

Product Documents for TLE1 Antibody (MSVA-856R) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for TLE1 Antibody (MSVA-856R) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for TLE1 Antibody (MSVA-856R) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review TLE1 Antibody (MSVA-856R) - Azide and BSA Free and earn rewards!

Have you used TLE1 Antibody (MSVA-856R) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies