Transcription factor E3 Antibody (1O9E9)

Novus Biologicals | Catalog # NBP3-15276

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1O9E9 expressed in HEK293
View all Transcription factor E3 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 191-440 of human Transcription factor E3 (P19532). LQQARRQQVKQYLSTTLGPKLASQALTPPPGPASAQPLPAPEAAHTTGPTGSAPNSPMALLTIGSSSEKEIDDVIDEIISLESSYNDEMLSYLPGGTTGLQLPSTLPVSGNLLDVYSSQGVATPAITVSNSCPAELPNIKREISETEAKALLKERQKKDNHNLIERRRRFNINDRIKELGTLIPKSSDPEMRWNKGTILKASVDYIRKLQKEQQRSKDLESRQRSLEQANRSLQLRIQELELQAQIHGLP

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Transcription factor E3 Antibody (1O9E9)

Western Blot: Transcription factor E3 Antibody (1O9E9) [NBP3-15276]

Western Blot: Transcription factor E3 Antibody (1O9E9) [NBP3-15276]

Western Blot: Transcription factor E3 Antibody (7V8M1) [NBP3-15276] - Western blot analysis of extracts of various cell lines, using Transcription factor E3 Rabbit mAb (NBP3-15276) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry-Paraffin: Transcription factor E3 Antibody (1O9E9) [NBP3-15276]

Immunohistochemistry-Paraffin: Transcription factor E3 Antibody (1O9E9) [NBP3-15276]

Immunohistochemistry-Paraffin: Transcription factor E3 Antibody (7V8M1) [NBP3-15276] - Immunohistochemistry of paraffin-embedded human esophageal cancer using Transcription factor E3 Rabbit mAb (NBP3-15276) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western Blot: Transcription factor E3 Antibody (1O9E9) [NBP3-15276]

Western Blot: Transcription factor E3 Antibody (1O9E9) [NBP3-15276]

Western Blot: Transcription factor E3 Antibody (7V8M1) [NBP3-15276] - Western blot analysis of extracts of HeLa cells, using Transcription factor E3 Rabbit mAb (NBP3-15276) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Transcription factor E3 Antibody (1O9E9)

Immunohistochemistry: Transcription factor E3 Antibody (1O9E9) [Transcription factor E3] -

Immunohistochemistry: Transcription factor E3 Antibody (1O9E9) [Transcription factor E3] - Immunohistochemistry analysis of paraffin-embedded Human acinar soft tissue sarcoma using Transcription factor E3 Rabbit mAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

Applications for Transcription factor E3 Antibody (1O9E9)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Transcription factor E3

TFE3, a member of the helix-loop-helix family of transcription factors, binds to the mu-E3 motif of the immunoglobulin heavy-chain enhancer and is expressed in many cell types (Henthorn et al., 1991 [PubMed 1685140]).[supplied by OMIM]

Alternate Names

BHLHE33, bHLHe33transcription factor E3, Class E basic helix-loop-helix protein 33, RCCP2, TFEA, transcription factor binding to IGHM enhancer 3, transcription factor E family, member A, Transcription factor for IgH enhancer

Gene Symbol

TFE3

Additional Transcription factor E3 Products

Product Documents for Transcription factor E3 Antibody (1O9E9)

Certificate of Analysis

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Product Specific Notices for Transcription factor E3 Antibody (1O9E9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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