TRIM29 Antibody (MSVA-629M)
Novus Biologicals | Catalog # NBP3-57258
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # MSVA-629M
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Product Specifications
Immunogen
Recombinant fragment (126 Amino acid residues between aa 1-200) of human TRIM29 protein Swissprot Q14134
Localization
Cytoplasmic and cell surface
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Description
This antibody is highly specific and optimized for use in Immunohistochemistry.
It has been validated on TMAs covering 76 normal tissues and numerous cancer tissues.
The antibody concentration is optimized for dilution within dilution range using commercially available antibody diluent for immunohistochemistry.
Positive Control
Postive control:Tonsil: A strong membranous and cytoplasmic TRIM29 immunostaining should be seen in squamous epithelial cells..
Negative Control
Negitve control:Tonsil: All non-epithelial cells must not show any TRIM29 immunostaining.
Storage
Antibody can be stored at 4degC. For extended storage, aliquot and store at -20degC. Avoid repeated freeze-thaw cycles.
Scientific Data Images for TRIM29 Antibody (MSVA-629M)
Immunohistochemistry-Paraffin:TRIM29 Antibody (MSVA-629M)NBP3-57258
Tonsil surface epithelium A strong membranous and cytoplasmic TRIM29 immunostaining is seen in squamous epithelial cells of the tonsil surface.
Immunohistochemistry-Paraffin:TRIM29 Antibody (MSVA-629M)NBP3-57258
Anal canal Squamous cell carcinoma with strong TRIM29 immunostaining of tumor cells.
Immunohistochemistry-Paraffin:TRIM29 Antibody (MSVA-629M)NBP3-57258
Prostate A moderate intensity predominantly membranous TRIM29 staining is seen in basal cells of the prostate.
TRIM29 Cancer Tissue Gallery
TRIM29 Cancer Tissue Gallery
TRIM29 Cancer Tissue Gallery
TRIM29 Cancer Tissue Gallery
TRIM29 Normal Tissue Gallery
TRIM29 Normal Tissue Gallery
TRIM29 Normal Tissue Gallery
TRIM29 Normal Tissue Gallery
TRIM29 Normal Tissue Gallery
TRIM29 Normal Tissue Gallery
Applications for TRIM29 Antibody (MSVA-629M)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:100 1:200
Application Notes
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS
Preservative
<0.1% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: TRIM29
Alternate Names
Ataxia telangiectasia group D-associated protein, ATDC, ATDC ataxia-telangiectasia group D-associated protein, FLJ36085, tripartite motif containing 29, tripartite motif protein TRIM29, tripartite motif-containing 29, tripartite motif-containing protein 29
Gene Symbol
TRIM29
Additional TRIM29 Products
Product Documents for TRIM29 Antibody (MSVA-629M)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for TRIM29 Antibody (MSVA-629M)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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