TROP-2 Antibody (MSVA-733R)

Novus Biologicals | Catalog # NBP3-57247

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # MSVA-733R
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Product Specifications

Immunogen

Recombinant fragment of human TACSTD2 protein (around aa 31-274) (exact sequence is proprietary) Swissprot P09758

Localization

Cell Surface

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody is highly specific and optimized for use in Immunohistochemistry.

It has been validated on TMAs covering 76 normal tissues and numerous cancer tissues.

The antibody concentration is optimized for dilution within dilution range using commercially available antibody diluent for immunohistochemistry.

Positive Control
Postive control:Liver: A strong membranous Trop-2 immunostaining should be seen in bile ducts..

Negative Control
Negitve control:Liver: Hepatocytes should not show any Trop-2 immunostaining.

Storage
Antibody can be stored at 4degC. For extended storage, aliquot and store at -20degC. Avoid repeated freeze-thaw cycles.

Scientific Data Images for TROP-2 Antibody (MSVA-733R)

TROP-2 Antibody (MSVA-733R)

Immunohistochemistry-Paraffin:TROP-2 Antibody (MSVA-733R)NBP3-57247

Kidney cortex In the kidney Trop 2 immunostaining is strong in distal tubuli and collecting ducts but weaker in the parietal layer of the Bowman capsule.
TROP-2 Antibody (MSVA-733R)

Immunohistochemistry-Paraffin:TROP-2 Antibody (MSVA-733R)NBP3-57247

Liver Strong Trop 2 staining in intrahepatic bile ducts of the liver.
TROP-2 Antibody (MSVA-733R)

Immunohistochemistry-Paraffin:TROP-2 Antibody (MSVA-733R)NBP3-57247

Strong Trop 2 immunostaining of a triple negative breast cancer of no special type NST.
TROP-2 Antibody (MSVA-733R) TROP-2 Antibody (MSVA-733R) TROP-2 Cancer Tissue Gallery

TROP-2 Cancer Tissue Gallery

TROP-2 Antibody (MSVA-733R) TROP-2 Antibody (MSVA-733R) TROP-2 Cancer Tissue Gallery

TROP-2 Cancer Tissue Gallery

TROP-2 Antibody (MSVA-733R) TROP-2 Antibody (MSVA-733R) TROP-2 Cancer Tissue Gallery

TROP-2 Cancer Tissue Gallery

TROP-2 Antibody (MSVA-733R) TROP-2 Antibody (MSVA-733R) TROP-2 Normal Tissue Gallery

TROP-2 Normal Tissue Gallery

TROP-2 Antibody (MSVA-733R) TROP-2 Antibody (MSVA-733R) TROP-2 Normal Tissue Gallery

TROP-2 Normal Tissue Gallery

TROP-2 Antibody (MSVA-733R) TROP-2 Antibody (MSVA-733R) TROP-2 Normal Tissue Gallery

TROP-2 Normal Tissue Gallery

TROP-2 Antibody (MSVA-733R) TROP-2 Antibody (MSVA-733R) TROP-2 Normal Tissue Gallery

TROP-2 Normal Tissue Gallery

TROP-2 Antibody (MSVA-733R) TROP-2 Antibody (MSVA-733R) TROP-2 Normal Tissue Gallery

TROP-2 Normal Tissue Gallery

Applications for TROP-2 Antibody (MSVA-733R)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:100 1:200
Application Notes
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Preservative

<0.1% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: TROP-2

TROP2 encodes a carcinoma-associated antigen defined by the monoclonal antibody GA733. This antigen is a member of a family including at least two type I membrane proteins. It transduces an intracellular calcium signal and acts as a cell surface receptor. Mutations of this gene result in gelatinous drop-like corneal dystrophy, an autosomal recessive disorder characterized by severe corneal amyloidosis leading to blindness. [provided by RefSeq]

Long Name

Tumor-associated Calcium Signal Transducer 2

Alternate Names

GA733-1, gp50, T16, TACSTD2, TROP2

Gene Symbol

TACSTD2

Additional TROP-2 Products

Product Documents for TROP-2 Antibody (MSVA-733R)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for TROP-2 Antibody (MSVA-733R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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