tropomyosin-4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-15146

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Zebrafish

Predicted:

Bovine (100%), Porcine (100%), Xenopus (92%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all tropomyosin-4 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human tropomyosin-4. The exact sequence is proprietary.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 24359494). Xenopus laevis (92%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for tropomyosin-4 Antibody - BSA Free

Western Blot: tropomyosin-4 Antibody [NBP2-15146]

Western Blot: tropomyosin-4 Antibody [NBP2-15146]

Western Blot: tropomyosin-4 Antibody [NBP2-15146] - Sample (30 ug of whole cell lysate) A: NT2D1 B: Hela 12% SDS PAGE; antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: tropomyosin-4 Antibody [NBP2-15146]

Immunohistochemistry-Paraffin: tropomyosin-4 Antibody [NBP2-15146]

Immunohistochemistry-Paraffin: tropomyosin-4 Antibody [NBP2-15146] - Paraffin-embedded 4T1 xenograft, using antibody at 1:100 dilution.
Western Blot: tropomyosin-4 Antibody [NBP2-15146]

Western Blot: tropomyosin-4 Antibody [NBP2-15146]

Western Blot: tropomyosin-4 Antibody [NBP2-15146] - Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE; antibody diluted at 1:1000.
tropomyosin-4 Antibody

Western Blot: tropomyosin-4 Antibody [NBP2-15146] -

Western Blot: tropomyosin-4 Antibody [NBP2-15146] - Sample (30 ug of whole cell lysate)
A: zebrafish heart
B: zebrafish muscle
12% SDS PAGE
NBP2-15146 diluted at 1:500
tropomyosin-4 Antibody

Immunohistochemistry: tropomyosin-4 Antibody [NBP2-15146] -

Immunohistochemistry: tropomyosin-4 Antibody [NBP2-15146] - tropomyosin-4 antibody detects tropomyosin-4 protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
tropomyosin-4 antibody (NBP2-15146) dilution: 1:100.

Applications for tropomyosin-4 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: tropomyosin-4

This gene encodes a member of the tropomyosin family of actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosins are dimers of coiled-coil proteins that polymerize end-to-end along the major groove in most actin filaments. They provide stability to the filaments and regulate access of other actin-binding proteins. In muscle cells, they regulate muscle contraction by controlling the binding of myosin heads to the actin filament. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

TM30p1, tropomyosin 4, tropomyosin alpha-4 chain, tropomyosin-4

Gene Symbol

TPM4

UniProt

P67936 (Human)

Additional tropomyosin-4 Products

Product Documents for tropomyosin-4 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for tropomyosin-4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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