TSLPR, also known as Delta and CRLM-2, is expressed on regulatory T cells (Treg), some subsets of helper T cells, innate lymphoid cells (ILC), monocytes, and dendritic cells. It associates with IL-7R alpha to form a high affinity receptor complex for epithelium-derived TSLP (thymic stromal lymphopoeitin). TSLP is a homeostatic cytokine that induces the release of T cell-attracting chemokines and enhances the maturation of CD1c+ dendritic cells.
![Western Blot: TSLPR/CRLF2 Antibody [NBP2-22384]](https://resources.rndsystems.com/images/products/TSLP-R-CRLF2-Antibody-Western-Blot-NBP2-22384-img0005.jpg "Western Blot: TSLPR/CRLF2 Antibody [NBP2-22384]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Mouse IgG2b Kappa
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Product Specifications
Immunogen
Amino acids 119-231 of human TSLPR protein were used as the immunogen for the antibody.
Clonality
Polyclonal
Host
Mouse
Isotype
IgG2b Kappa
Scientific Data Images for TSLPR/CRLF2 Antibody
Western Blot: TSLPR/CRLF2 Antibody [NBP2-22384]
Western Blot: TSLP R/CRLF2 Antibody [NBP2-22384] - Analysis of TSLPR in an A) partial recombinant protein (0.1 ug/ml) and B) human liver lysate (2ug/ml) using TSLPR antibody.![Immunohistochemistry-Paraffin: TSLPR/CRLF2 Antibody [NBP2-22384]](https://resources.rndsystems.com/images/products/TSLP-R-CRLF2-Antibody-Immunohistochemistry-Paraffin-NBP2-22384-img0006.jpg "Immunohistochemistry-Paraffin: TSLPR/CRLF2 Antibody [NBP2-22384]")
Immunohistochemistry-Paraffin: TSLPR/CRLF2 Antibody [NBP2-22384]
Immunohistochemistry-Paraffin: TSLP R/CRLF2 Antibody [NBP2-22384] - Paraffin-embedded normal human kidney tissue stained with TSLPR antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen.Applications for TSLPR/CRLF2 Antibody
Application
Recommended Usage
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
5-7 ug/ml
Western Blot
1-5 ug/ml
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TSLPR
Long Name
Thymic Stromal Lymphopoietin Receptor
Alternate Names
CRL2, CRLF2, CRLM-2, PCOR1, TSLP R
Gene Symbol
CRLF2
UniProt
Additional TSLPR Products
Product Documents for TSLPR/CRLF2 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for TSLPR/CRLF2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
