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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: TTVREQCEQLEKCVKARERLELCDERVSSRSHTEEDCTEELFDFLHARDHCVAHKLFNNLK
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for UQCRH Antibody - BSA Free
Immunohistochemistry-Paraffin: UQCRH Antibody [NBP1-93442]
Immunohistochemistry-Paraffin: UQCRH Antibody [NBP1-93442] - Staining of human liver shows moderate granular cytoplasmic positivity in hepatocytes.Immunohistochemistry-Paraffin: UQCRH Antibody [NBP1-93442]
Immunohistochemistry-Paraffin: UQCRH Antibody [NBP1-93442] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.Immunohistochemistry: UQCRH Antibody - BSA Free [NBP1-93442]
Staining of human liver shows moderate granular cytoplasmic positivity in hepatocytes.Immunohistochemistry: UQCRH Antibody - BSA Free [NBP1-93442]
Staining of human colon shows strong granular cytoplasmic positivity in glandular cells.Immunohistochemistry: UQCRH Antibody - BSA Free [NBP1-93442]
Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.Immunohistochemistry: UQCRH Antibody - BSA Free [NBP1-93442]
Staining of human heart muscle shows strong granular cytoplasmic positivity in cardiomyocytes.Applications for UQCRH Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Reviewed Applications
Read 2 reviews rated 2.5 using NBP1-93442 in the following applications:
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: UQCRH
Alternate Names
Complex III subunit 6, Complex III subunit VIII, cytochrome b-c1 complex subunit 6, mitochondrial, Cytochrome c1 non-heme 11 kDa protein, MGC111572, Mitochondrial hinge protein, QCR6, Ubiquinol-cytochrome c reductase complex 11 kDa protein, ubiquinol-cytochrome c reductase hinge protein, ubiquinol-cytochrome c reductase, complex III subunit VIII, UQCR8
Gene Symbol
UQCRH
Additional UQCRH Products
Product Documents for UQCRH Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for UQCRH Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Customer Reviews for UQCRH Antibody - BSA Free (2)
2.5 out of 5
2 Customer Ratings
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Application: Simple WesternSample Tested: human brain lysateSpecies: HumanVerified Customer | Posted 04/06/2023UQCRH Ab (C1) Antibodies NBP1-93442, ProteinSimple Western Blot on Jess Instrument; 3 and 1 microgram of human brain tissue lysate was tested with the antibodies diluted 10 times. No specific signal was detected at any exposure.Bio-Techne ResponseThis review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.
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Application: Western BlotSample Tested: HEK293a human embryonic kidney cell line, RCC4 Whole cell lysate, HEK293A whole cell lysate and 786O cell lysatesSpecies: HumanVerified Customer | Posted 08/09/2020Left 3 lanes: HEK293a, 786O, RCC4 whole cell lysate Right 3 lanes: HEK293a, 786O, RCC4 whole cell lysate with UQCRH overexpression
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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