VAMP3/Cellubrevin Antibody (4G9) - BSA Free

Novus Biologicals | Catalog # NBP1-97948

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Western Blot, ELISA, Flow Cytometry, Immunoprecipitation

Cited:

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 4G9

Format

BSA Free
View all VAMP3/Cellubrevin Primary Antibodies »

Product Specifications

Immunogen

Recombinant human VAMP3/Cellubrevin (1-77aa) purified from E. coli

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for VAMP3/Cellubrevin Antibody (4G9) - BSA Free

Western Blot: VAMP3/Cellubrevin Antibody (4G9)BSA Free [NBP1-97948]

Western Blot: VAMP3/Cellubrevin Antibody (4G9)BSA Free [NBP1-97948]

Western Blot: VAMP3/Cellubrevin Antibody (4G9) [NBP1-97948] - The mouse brain tissue lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human cellubrevin antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Flow Cytometry: VAMP3/Cellubrevin Antibody (4G9) - BSA Free [NBP1-97948]

Flow Cytometry: VAMP3/Cellubrevin Antibody (4G9) - BSA Free [NBP1-97948]

Flow Cytometry: VAMP3/Cellubrevin Antibody (4G9) [NBP1-97948] - Analysis of Cellubrevin in HeLa cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line)
Western Blot: VAMP3/Cellubrevin Antibody (4G9)BSA Free [NBP1-97948]

Western Blot: VAMP3/Cellubrevin Antibody (4G9)BSA Free [NBP1-97948]

Western Blot: VAMP3/Cellubrevin Antibody (4G9) [NBP1-97948] - Lane 1. 293T cell lysate Lane 2. Cellubrevin transfected 293T cell lysate.

Applications for VAMP3/Cellubrevin Antibody (4G9) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Western Blot

1:1000
Application Notes
Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Use in immunoprecipitation reported in scientific literature (PMID 27183589).

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VAMP3/Cellubrevin

Cellubrevin, also known as VAMP3, is a member of the Vamp/synaptobrevin family. Vamps, syntaxins, and the 25-kD synaptosomal-associated protein are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. Cellubrevin is a vesicular soluble N-ethylmaleimide sensitive factor attachment protein receptor (v-SNARE) homologous to the neuronal synaptobrevins 1/2 and is also a substrate of tetanus neurotoxin.

Alternate Names

CEBCellubrevin, cellubrevin, SYB3, synaptobrevin-3, VAMP-3, vesicle-associated membrane protein 3, vesicle-associated membrane protein 3 (cellubrevin)

Gene Symbol

VAMP3

UniProt

NP_004772 (Human)

Additional VAMP3/Cellubrevin Products

Product Documents for VAMP3/Cellubrevin Antibody (4G9) - BSA Free

Certificate of Analysis

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Product Specific Notices for VAMP3/Cellubrevin Antibody (4G9) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for VAMP3/Cellubrevin Antibody (4G9) - BSA Free

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Protocols

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FAQs

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