VEGF-C Antibody (HL2289) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25735

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL2289 expressed in HEK293

Format

Azide and BSA Free
View all VEGF-C Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment of human VEGF-C.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit VEGF-C Antibody (HL2289) - Azide and BSA Free (NBP3-25735) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for VEGF-C Antibody (HL2289) - Azide and BSA Free

VEGF-C Antibody (HL2289) - Azide and BSA Free

Western Blot: VEGF-C Antibody (HL2289) - Azide and BSA Free [NBP3-25735] -

Western Blot: VEGF-C Antibody (HL2289) - Azide and BSA Free [NBP3-25735] - U87-MG whole cell extract and conditioned medium (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with VEGFC antibody [HL2289] (NBP3-25735) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
VEGF-C Antibody (HL2289) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: VEGF-C Antibody (HL2289) - Azide and BSA Free [NBP3-25735] -

Immunocytochemistry/Immunofluorescence: VEGF-C Antibody (HL2289) - Azide and BSA Free [NBP3-25735] - VEGFC antibody [HL2289] detects VEGFC protein at cytoplasmic vesicles by immunofluorescent analysis. Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VEGFC stained by VEGFC antibody [HL2289] (NBP3-25735) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI.

Applications for VEGF-C Antibody (HL2289) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VEGF-C

Vascular endothelial growth factors (VEGFs), also known as vasculotropins, are a family of closely related growth factors having a conserved pattern of eight cysteine residues and sharing common VEGF receptors. VEGFs stimulate the proliferation of endothelial cells, induce angiogenesis, promote cell migration, increase vascular permeability, and inhibit apoptosis. The mitogenic activity of VEGFs appears to be mediated by specific VEGF receptors. The target cell specificity of VEGF is restricted to vascular endothelial cells. Vascular Endothelial Growth Factor C (VEGFC) is a member of the VEGF subfamily of PDGF-related growth factors. It is the ligand for Flt4 (VEGFR3) and KDR (VEGFR2). VEGFC binds Flt4 and induces tyrosine autophosphorylation of VEGFR3 and VEGFR2. VEGFC also stimulates the migration of bovine capillary endothelial cells in collagen gel. It is a specific growth factor for the lymphatic vascular system and mediates lymphangiogenesis. VEGFC is abundantly expressed in heart and skeletal muscle. Other tissues such as lung and kidney also express VEGFC. The human VEGFC gene is located on chromosome 4q34.

Long Name

Vascular Endothelial Growth Factor C

Alternate Names

VEGFC

Gene Symbol

VEGFC

Additional VEGF-C Products

Product Documents for VEGF-C Antibody (HL2289) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for VEGF-C Antibody (HL2289) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs for VEGF-C Antibody (HL2289) - Azide and BSA Free

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    • Q: I was wondering if you know of any blocking antibodies against IL-6 or VEGF-C that can be used in cell culture to quench these cytokines?
      A: We have no antibodies that are currently known to show blocking antibodies to VEGF-C. Please see this link to our IL-6 antibodies with blocking/neutralizing activity can be found at this link.
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