VIAAT/SLC32A1/VGAT Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-20857

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Predicted:

Rhesus Macaque (97%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all VIAAT/SLC32A1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human VIAAT/SLC32A1/VGAT. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (82%).

Localization

Cytoplasmic vesicle membrane; Multi-pass membrane protein

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for VIAAT/SLC32A1/VGAT Antibody - BSA Free

Immunohistochemistry-Frozen: VIAAT/SLC32A1/VGAT Antibody [NBP2-20857]

Immunohistochemistry-Frozen: VIAAT/SLC32A1/VGAT Antibody [NBP2-20857]

Immunohistochemistry-Frozen: VIAAT/SLC32A1/VGAT Antibody [NBP2-20857] - Frozen sectioned adult mouse retina. Green: VGAT protein stained by VGAT antibody [N1N2], N-term diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [11710] (NBP2-43559) diluted at 1:250. Blue: Fluoroshield with DAPI.
Immunohistochemistry-Paraffin: VIAAT/SLC32A1/VGAT Antibody [NBP2-20857]

Immunohistochemistry-Paraffin: VIAAT/SLC32A1/VGAT Antibody [NBP2-20857]

Immunohistochemistry-Paraffin: VIAAT/SLC32A1/VGAT Antibody [NBP2-20857] - CL1-5 xenograft, using VGAT antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
VIAAT/SLC32A1/VGAT Antibody

Immunohistochemistry-Paraffin: VIAAT/SLC32A1/VGAT Antibody [NBP2-20857] -

Immunohistochemistry-Paraffin: VIAAT/SLC32A1/VGAT Antibody [NBP2-20857] - VIAAT/SLC32A1/VGAT antibody [N1N2], N-term detects VIAAT/SLC32A1/VGAT protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: VIAAT/SLC32A1/VGAT stained by VIAAT/SLC32A1/VGAT antibody [N1N2], N-term (NBP2-20857) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.
Blue: Fluoroshield with DAPI.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
VIAAT/SLC32A1/VGAT Antibody - BSA Free

Western Blot: VIAAT/SLC32A1/VGAT Antibody [NBP2-20857] -

Non-transfected (-) and transfected (+) unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with VGAT antibody [N1N2], N-term (NBP2-20857) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
VIAAT/SLC32A1/VGAT Antibody - BSA Free

Western Blot: VIAAT/SLC32A1/VGAT Antibody [NBP2-20857] -

Boiled and unboiled various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with VGAT antibody [N1N2], N-term (NBP2-20857) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
VIAAT/SLC32A1/VGAT Antibody - BSA Free

Immunohistochemistry-Frozen: VIAAT/SLC32A1/VGAT Antibody [NBP2-20857] -

VGAT antibody [N1N2], N-term detects VGAT protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: VGAT protein stained by VGAT antibody [N1N2], N-term (NBP2-20857) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500.
Blue: Fluoroshield with DAPI.
VIAAT/SLC32A1/VGAT Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: VIAAT/SLC32A1/VGAT Antibody [NBP2-20857] -

VGAT antibody [N1N2], N-term detects VGAT protein by immunofluorescent analysis. Sample: DIV9 rat cortical neuron and Glia cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VGAT stained by VGAT antibody [N1N2], N-term (NBP2-20857) diluted at 1:250. Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500.
VIAAT/SLC32A1/VGAT Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: VIAAT/SLC32A1/VGAT Antibody [NBP2-20857] -

VGAT antibody [N1N2], N-term detects VGAT protein expression by immunofluorescent analysis.
Sample: Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: VGAT protein stained by VGAT antibody [N1N2], N-term (NBP2-20857) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:250.
Blue: Fluoroshield with DAPI.

Applications for VIAAT/SLC32A1/VGAT Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: VIAAT/SLC32A1

The protein encoded by this gene is an integral membrane protein involved in gamma-aminobutyric acid (GABA) and glycine uptake into synaptic vesicles. The encoded protein is a member of amino acid/polyamine transporter family II. [provided by RefSeq]

Long Name

Vesicular Inhibitory Amino Acid Transporter

Alternate Names

SLC32A1, VGAT

Gene Symbol

SLC32A1

UniProt

Q9H598 (Human)

Additional VIAAT/SLC32A1 Products

Product Documents for VIAAT/SLC32A1/VGAT Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for VIAAT/SLC32A1/VGAT Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Neuronal Markers

Citations for VIAAT/SLC32A1/VGAT Antibody - BSA Free

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Protocols

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