Viral Chikungunya Virus E1 Antibody Summary
Accession # Q8JUX5
This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Viral Chikungunya Virus E1 Monoclonal Antibody (Catalog # MAB97791).
This product is intended for assay development on various assay platforms requiring antibody pairs.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Viral Chikungunya Virus E1 ELISA Standard Curve. Recombinant Viral Chikungunya Virus E1 protein was serially diluted 2-fold and captured by Mouse Anti-Viral Chikungunya Virus E1 Monoclonal Antibody (Catalog # MAB97791) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Viral Chikungunya Virus E1 Monoclonal Antibody (Catalog # MAB9779) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Chikungunya Virus E1
Chikungunya fever is a pandemic disease caused by the flavivirus Chikungunya (CHIKV). The 47 kDa E1 glycoprotein mediates viral membrane fusion during CHIKV infection. The CHIKV envelope protein E1 is a component of the viral spike, which is composed of triplets of heterodimer of E1 and E2 glycoproteins, expressed on the viral surface. The viral spike proteins facilitate attachment to cell surfaces and viral entry into the cells. The E1 envelope protein is a class II fusion protein that mediates low pH-triggered membrane fusion during virus infection.
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