WASF1/WAVE1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-13180

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Chimpanzee (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all WASF1/WAVE1 Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse WASF1/WAVE1. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for WASF1/WAVE1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: WASF1/WAVE1 Antibody [NBP3-13180]

Immunocytochemistry/ Immunofluorescence: WASF1/WAVE1 Antibody [NBP3-13180]

Immunocytochemistry/Immunofluorescence: WASF1/WAVE1 Antibody [NBP3-13180] - WASF1/WAVE1 antibody detects WASF1/WAVE1 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: WASF1/WAVE1 stained by WASF1/WAVE1 antibody (NBP3-13180) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (NBP3-13505) diluted at 1:500. Blue: Fluoroshield with DAPI.
Immunohistochemistry-Paraffin: WASF1/WAVE1 Antibody [NBP3-13180]

Immunohistochemistry-Paraffin: WASF1/WAVE1 Antibody [NBP3-13180]

Immunohistochemistry-Paraffin: WASF1/WAVE1 Antibody [NBP3-13180] - WASF1/WAVE1 antibody detects WASF1/WAVE1 protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. WASF1/WAVE1 antibody (NBP3-13180) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: WASF1/WAVE1 Antibody [NBP3-13180]

Immunohistochemistry-Paraffin: WASF1/WAVE1 Antibody [NBP3-13180]

Immunohistochemistry-Paraffin: WASF1/WAVE1 Antibody [NBP3-13180] - WASF1/WAVE1 antibody detects WASF1/WAVE1 protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. WASF1/WAVE1 antibody (NBP3-13180) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
WASF1/WAVE1 Antibody - BSA Free

Western Blot: WASF1/WAVE1 Antibody [NBP3-13180] -

Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with WAVE1 antibody (NBP3-13180) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
WASF1/WAVE1 Antibody - BSA Free

Western Blot: WASF1/WAVE1 Antibody [NBP3-13180] -

Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with WAVE1 antibody (NBP3-13180) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
WASF1/WAVE1 Antibody - BSA Free

Western Blot: WASF1/WAVE1 Antibody [NBP3-13180] -

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with WAVE1 antibody (NBP3-13180) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
WASF1/WAVE1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: WASF1/WAVE1 Antibody [NBP3-13180] -

WAVE1 antibody detects WAVE1 protein at cytoplasm by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: WAVE1 protein stained by WAVE1 antibody (NBP3-13180) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.

Applications for WASF1/WAVE1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: WASF1/WAVE1

WASP (for Wiskott-Aldrich syndrome protein), N-WASP are downstream effectors of Cdc42 that are implicated in actin polymerization and cytoskeletal organization. The WASP family also includes VASP (vasodilator-stimulated phosphoprotein) and Mena (for mammalian enabled protein), which accumulate at focal adhesions and are also involved in the regulation of the actin cytoskeleton. The WAVE proteins are related to the WASP family proteins and are likewise involved in mediating actin reorganization downstream of the Rho family of small GTPases. The two protein homologs WAVE1 and WAVE2 specifically regulate membrane ruffling by inducing the formation of actin filament clusters in response to GTP binding and activating Rac. The WAVE proteins mediate this actin polymerization by cooperating with the Arp2/3 complex, a nucleation core, and thereby promoting the formation of actin filaments. WAVE1, which is also designated SCAR (for suppressor of cAR), is expressed primarily in the brain, while WAVE2 is widely expressed with the expression highest in peripheral blood leukocytes.

Long Name

Wiskott-Aldrich Syndrome Protein Family Member 1

Alternate Names

SCAR1, WASP1, WAVE1

Gene Symbol

WASF1

Additional WASF1/WAVE1 Products

Product Documents for WASF1/WAVE1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for WASF1/WAVE1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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