WBP11 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-20886

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (99%), Rhesus Macaque (99%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all WBP11 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human WBP11. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (84%).

Localization

Nucleus; Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for WBP11 Antibody - BSA Free

Immunohistochemistry-Paraffin: WBP11 Antibody [NBP2-20886]

Immunohistochemistry-Paraffin: WBP11 Antibody [NBP2-20886]

Immunohistochemistry-Paraffin: WBP11 Antibody [NBP2-20886] - Paraffin-embedded rat brain. WBP11 antibodydiluted at 1:500.
Immunohistochemistry-Paraffin: WBP11 Antibody [NBP2-20886]

Immunohistochemistry-Paraffin: WBP11 Antibody [NBP2-20886]

Immunohistochemistry-Paraffin: WBP11 Antibody [NBP2-20886] - Immunohistochemical analysis of paraffin-embedded SkHep1 xenograft, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: WBP11 Antibody [NBP2-20886]

Immunohistochemistry-Paraffin: WBP11 Antibody [NBP2-20886]

Immunohistochemistry-Paraffin: WBP11 Antibody [NBP2-20886] - Paraffin-embedded mouse brain. WBP11 antibody diluted at 1:500.
WBP11 Antibody

Western Blot: WBP11 Antibody [NBP2-20886] -

Western Blot: WBP11 Antibody [NBP2-20886] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with WBP11 antibody (NBP2-20886) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
WBP11 Antibody - BSA Free

Immunoprecipitation: WBP11 Antibody [NBP2-20886] -

WBP11 antibody immunoprecipitates WBP11 protein in IP experiments.
IP samples: Jurkat whole cell extract
A. Control with 4 ug of preimmune Rabbit IgG
B. Immunoprecipitation of WBP11 protein by 4 ug WBP11 antibody (NBP2-20886)
7.5 % SDS-PAGE
The immunoprecipitated WBP11 protein was detected by WBP11 antibody (NBP2-20886) diluted at 1:1000.
[EasyBlot anti-rabbit IgG was used as a secondary reagent]
WBP11 Antibody - BSA Free

Immunohistochemistry-Paraffin: WBP11 Antibody [NBP2-20886] -

WBP11 antibody detects WBP11 protein at nucleus in rat adrenal by immunohistochemical analysis.
Sample: Paraffin-embedded rat adrenal.
WBP11 antibody (NBP2-20886) diluted at 1:500.
br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for WBP11 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:1000-1:10000

Reviewed Applications

Read 1 review rated 5 using NBP2-20886 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: WBP11

This gene encodes a nuclear protein, which colocalizes with mRNA splicing factors and intermediate filament-containing perinuclear networks. This protein has 95% amino acid sequence identity to the mouse Wbp11 protein. It contains two proline-rich regions that bind to the WW domain of Npw38, a nuclear protein, and thus this protein is also called Npw38-binding protein NpwBP. The Npw38-NpwBP complex may function as a component of an mRNA factory in the nucleus. [provided by RefSeq]

Alternate Names

DKFZp779M1063, Npw38-binding protein, Npw38-binding protein NpwBP, NpwBP, NPWBPsplicing factor, PQBP1 and PP1 interacting, SH3 domain-binding protein SNP70, SIPP1WW domain-binding protein 11, SNP70, Splicing factor that interacts with PQBP-1 and PP1, WBP-11, WW domain binding protein 11

Gene Symbol

WBP11

UniProt

Q9Y2W2 (Human)

Additional WBP11 Products

Product Documents for WBP11 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for WBP11 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for WBP11 Antibody - BSA Free (1)

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Hela whole cell lysate
    Species: Human
    Verified Customer | Posted 06/06/2015
    Hela cells lysate 25 ug protein loaded on SDS gel (4%-12%) used MES for electrophoresis. Major band comes ~95 kDa
    WBP11 Antibody - BSA Free NBP2-20886

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Protocols

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