Wnt16 Antibody (HL1498) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25751

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1498 expressed in HEK293

Format

Azide and BSA Free
View all Wnt16 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Wnt16. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Wnt16 Antibody (HL1498) - Azide and BSA Free

Wnt16 Antibody (HL1498) - Azide and BSA Free

Immunohistochemistry-Paraffin: Wnt16 Antibody (HL1498) - Azide and BSA Free [NBP3-25751] -

Immunohistochemistry-Paraffin: Wnt16 Antibody (HL1498) - Azide and BSA Free [NBP3-25751] - Wnt16 antibody [HL1498] detects secreted Wnt16 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Wnt16 stained by Wnt16 antibody [HL1498] (NBP3-25751) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Wnt16 Antibody (HL1498) - Azide and BSA Free

Western Blot: Wnt16 Antibody (HL1498) - Azide and BSA Free [NBP3-25751] -

Western Blot: Wnt16 Antibody (HL1498) - Azide and BSA Free [NBP3-25751] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt16 antibody [HL1498] (NBP3-25751) diluted at 1:5000. The HRP-conjugated anti-FALSE was used to detect the primary antibody.
Wnt16 Antibody (HL1498) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: Wnt16 Antibody (HL1498) - Azide and BSA Free [NBP3-25751] -

Immunocytochemistry/Immunofluorescence: Wnt16 Antibody (HL1498) - Azide and BSA Free [NBP3-25751] - Wnt16 antibody [HL1498] detects Wnt16 protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Wnt16 stained by Wnt16 antibody [HL1498] (NBP3-25751) diluted at 1:500. Blue: Fluoroshield with DAPI.

Applications for Wnt16 Antibody (HL1498) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Wnt16

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It contains two transcript variants diverging at the 5' termini. These two variants are proposed to be the products of separate promoters and not to be splice variants from a single promoter. They are differentially expressed in normal tissues, one of which (variant 2) is expressed at significant levels only in the pancreas, whereas another one (variant 1) is expressed more ubiquitously with highest levels in adult kidney, placenta, brain, heart, and spleen. [provided by RefSeq]. Transcript Variant: This variant (2) differs from variant 1 at the 5' terminus including 5' UTR and the coding region for the N-terminus. It encodes a shorter isoform than variant 1.

Alternate Names

protein Wnt-16, wingless-type MMTV integration site family, member 16

Gene Symbol

WNT16

Additional Wnt16 Products

Product Documents for Wnt16 Antibody (HL1498) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Wnt16 Antibody (HL1498) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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