ZIM2 Antibody

Novus Biologicals | Catalog # NBP1-80590

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
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Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids:SSNVREYQKARAKKKYIEHRSNETSVIHSLPFGEQTFRPRGMLYECQECGECFAHSSDLTEHQKIHDREKPSGSRNYEWSVIRSLAPTDPQTSYAQEQYAKEQARNKCKDFRQFFATSEDLNTNQKIYDQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ZIM2 Antibody

Western Blot: 0 [NBP1-80590]

Western Blot: 0 [NBP1-80590]

Western Blot: ZIM2 Antibody [NBP1-80590] - Analysis in human cell line BEWO.
Immunocytochemistry/ Immunofluorescence: ZIM2 Antibody [NBP1-80590]

Immunocytochemistry/ Immunofluorescence: ZIM2 Antibody [NBP1-80590]

Immunocytochemistry/Immunofluorescence: ZIM2 Antibody [NBP1-80590] - Staining of human cell line Hep G2 shows localization to nucleoplasm.
ZIM2 Antibody Immunohistochemistry: ZIM2 Antibody [NBP1-80590]

Immunohistochemistry: ZIM2 Antibody [NBP1-80590]

Staining of human placenta shows strong nuclear positivity in trophoblastic cells.

Applications for ZIM2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ZIM2

In human, ZIM2 and PEG3 (GeneID:5178) are two distinct genes that share a set of 5' exons and have a common promoter, and both genes are paternally expressed (PMID:15203203). Alternative splicing events connect the shared exons either with the remaining 4 exons unique to ZIM2, or with the remaining 2 exons unique to PEG3. This is in contrast to mouse and cow, where ZIM2 and PEG3 genes do not share exons in common, and the imprinting status of ZIM2 is also not conserved amongst mammals. Additional 5' alternatively spliced transcripts encoding the same protein have been found for the human ZIM2 gene. (provided by RefSeq)

Alternate Names

zinc finger imprinted 2, zinc finger, imprinted 2, ZNF656Zinc finger protein 656

Gene Symbol

ZIM2

Additional ZIM2 Products

Product Documents for ZIM2 Antibody

Certificate of Analysis

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Product Specific Notices for ZIM2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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