ZNF18 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-81758

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ISIQVLGQDILSEKMESPSCQVGEVEPHLEVVPQELGLENSSSGPGELLSHIVKEESDTEAELALAASQPARLEERLIRDQDLGASLLPAAPQEQWRQLDSTQKEQYWDLMLETYGKMVSGAGISHPKSDLTNS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ZNF18 Antibody - BSA Free

Western Blot: ZNF18 Antibody [NBP1-81758]

Western Blot: ZNF18 Antibody [NBP1-81758]

Western Blot: ZNF18 Antibody [NBP1-81758] - Analysis in control (vector only transfected HEK293T lysate) and ZNF18 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: ZNF18 Antibody [NBP1-81758]

Immunocytochemistry/ Immunofluorescence: ZNF18 Antibody [NBP1-81758]

Immunocytochemistry/Immunofluorescence: ZNF18 Antibody [NBP1-81758] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, plasma membrane, cytosol & the Golgi apparatus.
Immunohistochemistry: ZNF18 Antibody [NBP1-81758]

Immunohistochemistry: ZNF18 Antibody [NBP1-81758]

Immunohistochemistry: ZNF18 Antibody [NBP1-81758] - Staining of human stomach shows strong cytoplasmic and nuclear positivity in glandular cells.
ZNF18 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: ZNF18 Antibody - BSA Free [NBP1-81758]

Chromatin Immunoprecipitation-exo-Seq: ZNF18 Antibody - BSA Free [NBP1-81758]

ChIP-Exo-Seq composite graph for Anti-ZNF18 (NBP1-81758) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.
ZNF18 Antibody - BSA Free ZNF18 Antibody [NBP1-81758] -

ZNF18 Antibody [NBP1-81758] -

Staining of human testis shows strong cytoplasmic and nuclear positivity in cells in seminiferous ducts.
ZNF18 Antibody - BSA Free

ZNF18 Antibody [NBP1-81758] -

Staining of human cerebral cortex shows strong cytoplasmic-nuclear positivity in neurons.
ZNF18 Antibody - BSA Free

ZNF18 Antibody [NBP1-81758] -

Staining of human lymph node shows strong nuclear positivity in non-germinal center cells and germinal center cells.
ZNF18 Antibody - BSA Free

ZNF18 Antibody [NBP1-81758] -

Staining of human colon shows moderate nuclear positivity in glandular cells.

Applications for ZNF18 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ZNF18

ZNF18 may be involved in transcriptional regulation.

Alternate Names

HDSG1Zinc finger protein 535, heart development-specific protein, KOX11Heart development-specific gene 1 protein, Zfp535, zinc finger protein 18, zinc finger protein 18 (KOX 11), ZKSCAN6Zinc finger protein KOX11, ZNF535Zinc finger protein with KRAB and SCAN domains 6

Entrez Gene IDs

7566 (Human)

Gene Symbol

ZNF18

UniProt

Additional ZNF18 Products

Product Documents for ZNF18 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for ZNF18 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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