Single Cell Dispensing Resources
Videos | Application Notes | Webinars | Brochures | User Guides | FAQs
Tools
Unlocking Innovation from a Single Cell-Automation for Cell Line Development
Identify More Diverse Cell Types in 10X Genomics Workflows
Empower CRISPR Cell Engineering with Innovative Single Cell Sorting
Pala Single Cell Sorter and Dispenser Brochure
Pala User Guide
Pala Quick Reference Guide
FAQs
What conditions will my cells be subject to in using the Pala?
Unlike conventional FACS that relies on high pressure to push the sheath and sample to form a consistent stream in the flow chamber, Pala single cell dispensers utilize microfluidics, on which a gentle positive pressure and negative pressure (20-30 times lower than that of FACS) are applied to guide the flow of cells. The entire microfluidics path where the sample is in contact is contained in the disposable cell cartridge and hence the amount of time the cells are exposed to the pressure is also much shorter than FACS.
What is the maintenance required for the R&D Systems single cell dispensers?
It is recommended for the user to flush the system with sterile ddH2O using the automated shutdown procedure after each use. If the instrument is going to be sitting for an extended period with no use, it is recommended to empty the sheath bottle and perform a shutdown to dry out the fluidics lines and empty the waste bottle prior to storage. Depending on what sheath buffers are used, our FAS may make recommendations to adjust the maintenance protocol. A software guided sterilization procedure that takes about 60 minutes is strongly recommended upon bringing the instrument into a sterile environment
(e.g. tissue culture hood). Maintaining sterility of the instrument in a sterile hood follows typical aseptic techniques such as running 70% ethanol through the system for 5 minutes followed by sterile cell culture grade water at weekly or semi-monthly intervals.
What is the size limit of cells that the Pala Single Cell Sorter & Dispenser can sort?
The cartridge flow chamber where cells travel is 50 microns x 150 microns and is designed to handle most mammalian cell types. Cells must be dissociated into single cells and aggregates must be removed prior to loading the cell cartridge to prevent clogging. Additionally, cell concentrations may need to be adjusted depending on the size of the cell. We have demonstrated successful isolation with nuclei, a variety of mammalian cell types, and larger 30 micron hydrogels.
Does the Pala Single Cell Sorter & Dispenser require special sheath fluid?
No special sheath fluid is required. For single cell dispensing mode, water or PBS or culture medium of choice can be used. For bulk sorting, PBS or culture medium of choice must be used.
Can cartridges be reused?
To ensure sterile conditions and prevent sample-to-sample carryover, it is best that new cartridges are used for each experiment. Reloading the cartridge with more cells of the same cartridge can be performed but should be limited to the day of use. Using the same cartridge across multiple days is not recommended.