14-3-3 epsilon Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-89827

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MDDREDLVYQAKLAEQAERYDEMVESMKKVAGMDVELTVEERNLLSVAYKNVIGARRASWRIISSIEQKE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for 14-3-3 epsilon Antibody - BSA Free

Immunohistochemistry-Paraffin: 14-3-3 epsilon Antibody [NBP1-89827]

Immunohistochemistry-Paraffin: 14-3-3 epsilon Antibody [NBP1-89827]

Immunohistochemistry-Paraffin: 14-3-3 epsilon Antibody [NBP1-89827] - Staining of human cerebral cortex shows strong nuclear and cytoplasmic positivity in neuronal cells.
14-3-3 epsilon Antibody - BSA Free Western Blot: 14-3-3 epsilon Antibody - BSA Free [NBP1-89827]

Western Blot: 14-3-3 epsilon Antibody - BSA Free [NBP1-89827]

Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
14-3-3 epsilon Antibody - BSA Free Western Blot: 14-3-3 epsilon Antibody - BSA Free [NBP1-89827]

Western Blot: 14-3-3 epsilon Antibody - BSA Free [NBP1-89827]

Analysis in human cell line SCLC-21H.

Applications for 14-3-3 epsilon Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reviewed Applications

Read 1 review rated 5 using NBP1-89827 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: 14-3-3 epsilon

14-3-3 epsilon product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer.

Alternate Names

1433 epsilon, YWHAE

Gene Symbol

YWHAE

Additional 14-3-3 epsilon Products

Product Documents for 14-3-3 epsilon Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for 14-3-3 epsilon Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for 14-3-3 epsilon Antibody - BSA Free

Customer Reviews for 14-3-3 epsilon Antibody - BSA Free (1)

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  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: Rat primary motor neurons, plated on PDL-coated coverslips
    Species: Rat
    Verified Customer | Posted 09/24/2015
    Rat Motor neurons, fixed in Methanol, stained with 14-3-3e (1:200). Nuclei were stained with DAPI
    14-3-3 epsilon Antibody - BSA Free NBP1-89827

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Protocols

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FAQs

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