14-3-3 sigma/Stratifin Antibody (CPTC-SFN-2)

Novus Biologicals | Catalog # NBP3-07326

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # CPTC-SFN-2
View all 14-3-3 sigma Primary Antibodies »

Product Specifications

Immunogen

Recombinant human full-length 14-3-3 sigma/Stratifin protein (Uniprot: P31947)

Localization

Cytoplasm and nucleus. May be secreted by a non-classical secretory pathway.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08214)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for 14-3-3 sigma/Stratifin Antibody (CPTC-SFN-2)

Western Blot: 14-3-3 sigma/Stratifin Antibody (CPTC-SFN-2) [NBP3-07326]

Western Blot: 14-3-3 sigma/Stratifin Antibody (CPTC-SFN-2) [NBP3-07326]

Western Blot: 14-3-3 sigma/Stratifin Antibody (CPTC-SFN-2) [NBP3-07326] - Western Blot Analysis of Human PC-3 cell lysate using 14-3-3 sigma/Stratifin Mouse Monoclonal Antibody (CPTC-SFN-2).
Immunohistochemistry-Paraffin: 14-3-3 sigma/Stratifin Antibody (CPTC-SFN-2) [NBP3-07326]

Immunohistochemistry-Paraffin: 14-3-3 sigma/Stratifin Antibody (CPTC-SFN-2) [NBP3-07326]

Immunohistochemistry-Paraffin: 14-3-3 sigma/Stratifin Antibody (CPTC-SFN-2) [NBP3-07326] - Formalin-fixed, paraffin-embedded human Skin stained with 14-3-3 sigma/Stratifin Mouse Monoclonal Antibody (CPTC-SFN-2).
Protein Array: 14-3-3 sigma/Stratifin Antibody (CPTC-SFN-2) [NBP3-07326]

Protein Array: 14-3-3 sigma/Stratifin Antibody (CPTC-SFN-2) [NBP3-07326]

Protein Array: 14-3-3 sigma/Stratifin Antibody (CPTC-SFN-2) [NBP3-07326] - Analysis of Protein Array containing more than 19,000 full-length human proteins using 14-3-3 sigma/Stratifin Mouse Monoclonal Antibody (CPTC-SFN-2).

Applications for 14-3-3 sigma/Stratifin Antibody (CPTC-SFN-2)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: 14-3-3 sigma

14-3-3 sigma was identified as an epithelial cell marker. It is part of the 14-3-3 family of proteins in which seven isoforms have been identified: beta, zeta, gamma, eta, epsilon, tau, and sigma. 14-3-3 proteins function as adaptors that bind with a number of partners to mediate various signaling pathways. 14-3-3 sigma is also called stratifin or HME1 and appears to function as a tumor suppressor whose expression can be downregulated via methylation. Loss of 14-3-3 sigma expression results in a defective G2/M phase checkpoint and appears to contribute to both epithelial and non-epithelial tumorigenesis. Alternate names for 14-3-3 sigma include epithelial marker protein 1, SFN and YWHAS.

Alternate Names

1433 sigma, Epithelial cell marker protein 1, SFN, YWHAS

Gene Symbol

SFN

UniProt

P31947

Additional 14-3-3 sigma Products

Product Documents for 14-3-3 sigma/Stratifin Antibody (CPTC-SFN-2)

Certificate of Analysis

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Product Specific Notices for 14-3-3 sigma/Stratifin Antibody (CPTC-SFN-2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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