14-3-3 zeta Antibody - Azide Free

Novus Biologicals | Catalog # NBP1-31325

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Chicken (99%), Porcine (99%), Rat (99%), Rhesus Macaque (100%), Sheep (99%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide Free
View all 14-3-3 zeta Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human 14-3-3 zeta. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (89%).

Localization

Cytoplasm, Melanosome

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for 14-3-3 zeta Antibody - Azide Free

Western Blot: 14-3-3 zeta Antibody [NBP1-31325]

Western Blot: 14-3-3 zeta Antibody [NBP1-31325]

Western Blot: 14-3-3 zeta Antibody [NBP1-31325] - Sample(30 ug of whole cell lysate) A:Hep G2 B:MOLT4 12% SDS PAGE, antibody diluted at 1:2000.
Immunohistochemistry-Paraffin: 14-3-3 zeta Antibody [NBP1-31325]

Immunohistochemistry-Paraffin: 14-3-3 zeta Antibody [NBP1-31325]

Immunohistochemistry-Paraffin: 14-3-3 zeta Antibody [NBP1-31325] - Mouse colon. 14-3-3 zeta antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: 14-3-3 zeta Antibody [NBP1-31325]

Immunohistochemistry-Paraffin: 14-3-3 zeta Antibody [NBP1-31325]

Immunohistochemistry-Paraffin: 14-3-3 zeta Antibody [NBP1-31325] - Cal27 xenograft, using 14-3-3 zeta antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
14-3-3 zeta Antibody

Western Blot: 14-3-3 zeta Antibody [NBP1-31325] -

Western Blot: 14-3-3 zeta Antibody [NBP1-31325] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 zeta antibody (NBP1-31325) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
14-3-3 zeta Antibody

Western Blot: 14-3-3 zeta Antibody [NBP1-31325] -

Western Blot: 14-3-3 zeta Antibody [NBP1-31325] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 zeta antibody (NBP1-31325) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
14-3-3 zeta Antibody - Azide Free

Western Blot: 14-3-3 zeta Antibody [NBP1-31325] -

Sample (30 ug of whole cell lysate)
A:NIH-3T3
12% SDS PAGE
NBP1-31325 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for 14-3-3 zeta Antibody - Azide Free

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Format

Azide Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: 14-3-3 zeta

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Two transcript variants differing in the 5' UTR, but encoding the same protein, have been identified for this gene. [provided by RefSeq]

Alternate Names

1433 zeta, KCIP-1, YWHAZ

Gene Symbol

YWHAZ

Additional 14-3-3 zeta Products

Product Documents for 14-3-3 zeta Antibody - Azide Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for 14-3-3 zeta Antibody - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for 14-3-3 zeta Antibody - Azide Free

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Protocols

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