5-HT1E Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-90320

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all 5-HT1E Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: RIYHAAKSLYQKRGSSRHLSNRSTDSQNSFASCKLTQTFCVSDFSTSDPTTEFEKFHASIRIPPFDNDLDHPGERQQISSTRER

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for 5-HT1E Antibody - BSA Free

Immunohistochemistry-Paraffin: 5-HT1E Antibody [NBP1-90320]

Immunohistochemistry-Paraffin: 5-HT1E Antibody [NBP1-90320]

Immunohistochemistry-Paraffin: 5-HT1E Antibody [NBP1-90320] - Staining of human cerebral cortex shows moderate positivity in neuropil.
5-HT1E Antibody - BSA Free Immunohistochemistry: 5-HT1E Antibody - BSA Free [NBP1-90320]

Immunohistochemistry: 5-HT1E Antibody - BSA Free [NBP1-90320]

Staining of human testis shows moderate membranous positivity in cells in seminiferous ducts.
5-HT1E Antibody - BSA Free Immunohistochemistry: 5-HT1E Antibody - BSA Free [NBP1-90320]

Immunohistochemistry: 5-HT1E Antibody - BSA Free [NBP1-90320]

Staining of human skeletal muscle shows no positivity in myocytes as expected.
5-HT1E Antibody - BSA Free Immunohistochemistry: 5-HT1E Antibody - BSA Free [NBP1-90320]

Immunohistochemistry: 5-HT1E Antibody - BSA Free [NBP1-90320]

Staining of human endometrium shows moderate membranous positivity in glandular cells.
5-HT1E Antibody - BSA Free Western Blot: 5-HT1E Antibody - BSA Free [NBP1-90320]

Western Blot: 5-HT1E Antibody - BSA Free [NBP1-90320]

Analysis in control (vector only transfected HEK293T lysate) and HTR1E over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).

Applications for 5-HT1E Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: 5-HT1E

5HT1E Receptor, also known as 5-hydroxytryptamine receptor 1E, is a 365 amino acid protein that is 42 kDa, belongs to the G-protein coupled receptor 1 family, has cell membrane intracellular location; commonly found in the cortex, caudate putamen, claustrum, hippocampus, and amygdala; is one of the many different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that plays a role of a neurotransmitter, a hormone, and a mitogen; this receptor activity is mediated by G proteins that inhibit adenylate cyclase activity. The protein is being studied for its involvement in attention deficit hyperactivity disorder, autism spectrum disorder, chronic fatigue syndrome, hermaphroditism, pharyngitis, neuronitis, and skin papilloma. This protein has been linked to the GPCR downstream signaling, GPCR ligand binding, class A/1 (Rhodopsin-like receptors), serotonin receptors, amine ligand-binding receptors, intracellular calcium signaling, signal transduction, G alpha (i) signaling events, neuroactive ligand-receptor interaction, and amine ligand-binding receptors pathways where it interacts with PSMC3, PSMC4, ADORA3, ADORA1, ADRA2A, APP, CCR3 and over 100 other proteins.

Long Name

5-Hydroxytryptamine Receptor 1E

Alternate Names

5HT1E, HTR1E, S31

Entrez Gene IDs

3354 (Human)

Gene Symbol

HTR1E

UniProt

P28566 (Human)

Additional 5-HT1E Products

Product Documents for 5-HT1E Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for 5-HT1E Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for 5-HT1E Antibody - BSA Free

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Protocols

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