5-HT3A Antibody

Novus Biologicals | Catalog # NB100-41382

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Peptide ELISA, Flow Cytometry

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all 5-HT3A Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-GPQDFEKSPRDR corresponding to internal region according to NP_998786.2, NP_000860.2, NP_001155244.1.

Reactivity Notes

Mouse reactivity reported from a verified customer review.

Specificity

This antibody is expected to recognise all three reported isoforms (NP_998786.2; NP_000860.2; NP_001155244.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for 5-HT3A Antibody

Immunohistochemistry-Paraffin: 5-HT3A Antibody [NB100-41382]

Immunohistochemistry-Paraffin: 5-HT3A Antibody [NB100-41382]

Immunohistochemistry-Paraffin: 5-HT3A Antibody [NB100-41382] - Staining of human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Antibody at 3.75 ug/mL.
Flow Cytometry: 5-HT3A Antibody [NB100-41382]

Flow Cytometry: 5-HT3A Antibody [NB100-41382]

Flow Cytometry: 5-HT3A Antibody [NB100-41382] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Immunohistochemistry-Frozen: 5-HT3A Antibody [NB100-41382]

Immunohistochemistry-Frozen: 5-HT3A Antibody [NB100-41382]

Immunohistochemistry-Frozen: 5-HT3A Antibody [NB100-41382] - Analysis of 5-HT3A in C57BL/6 mouse retina section using anti-5-HT3A antibody. IHC-Fr image submitted by a verified customer review.

Applications for 5-HT3A Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunohistochemistry-Paraffin

3.75 ug/mL

Peptide ELISA

Detection limit 1:128000
Application Notes
This 5-HT3A Antibody is validated for IHC-Frozen from a verified customer review.

Reviewed Applications

Read 1 review rated 4 using NB100-41382 in the following applications:

Flow Cytometry Panel Builder

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: 5-HT3A

The product of this gene belongs to the ligand-gated ion channel receptor superfamily. This gene encodes subunit A of the type 3 receptor for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor causes fast, depolarizing responses in neurons after activation. It appears that the heteromeric combination of A and B subunits is necessary to provide the full functional features of this receptor, since either subunit alone results in receptors with very low conductance and response amplitude. Alternatively spliced transcript variants encoding different isoforms have been identified.

Long Name

5-Hydroxytryptamine Receptor 3A

Alternate Names

5HT3A, HTR3A

Gene Symbol

HTR3A

UniProt

NP_998786.1 (Human)

Additional 5-HT3A Products

Product Documents for 5-HT3A Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for 5-HT3A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for 5-HT3A Antibody

Customer Reviews for 5-HT3A Antibody (1)

4 out of 5
1 Customer Rating
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Customer Images

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  • Name: Renee Ryals
    Application: Immunohistochemistry-Frozen
    Sample Tested: C57BL/6 mouse retina
    Species: Mouse
    Verified Customer | Posted 03/03/2015
    Confocal image of retinal section stained with NB100-41382 at 1:400 dilution.
    5-HT3A Antibody NB100-41382

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