5-HT7 Antibody

Novus Biologicals | Catalog # NBP1-44051

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all 5-HT7 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence QNADYCRKKGHDS corresponding to C-Terminus according to NP_000863.1.

Epitope

C-Terminus

Specificity

This antibody is expected to recognize isoform a (NP_000863.1) only.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for 5-HT7 Antibody

Western Blot: 5-HT7 Antibody [NBP1-44051]

Western Blot: 5-HT7 Antibody [NBP1-44051]

Western Blot: 5-HT7 Antibody [NBP1-44051] - (1ug/ml) Analysis of KELLY lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for 5-HT7 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:16000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 50 kDa band observed in KELLY lysate (calculated MW of 49.5 kDa band according to NP_000863.1). An additional band of unknown identity was also consistently observed at 37 kDa. This band was successfully blocked by incubation with the immunizing peptide.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: 5-HT7

Receptors for serotonin (5-hydroxytryptamine, 5-HT) are classified into seven major classes (5-HTR1-7), based on structural, functional and pharmacological criteria (Hoyer et al, 1994). The 5-HT7 receptor (5-HT7R) is a seven-transmembrane-domain G-protein-coupled receptor that has important roles in regulating diverse biological process in the central and peripheral nervous systems (reviewed in Hedlund and Sutcliffe, 2004). Receptors for serotonin (5-hydroxytryptamine, 5-HT) are classified into seven major classes (5-HT1-7), based on structural, functional and pharmacological criteria (Hoyer et al, 1994). Sequence alignment shows a high degree of interspecies 5-HT7R homology (>90%), and a low homology with other 5-HTRs (

Long Name

5-Hydroxytryptamine Receptor 7

Alternate Names

5-HT-X, 5HT7, HTR7

Entrez Gene IDs

3363 (Human)

Gene Symbol

HTR7

UniProt

NP_000863.1 (Human)

Additional 5-HT7 Products

Product Documents for 5-HT7 Antibody

Certificate of Analysis

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Product Specific Notices for 5-HT7 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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