Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Bovine (94%), Mouse (93%), Rat (92%). Backed by our 100% Guarantee.

Applications

Validated:

Multiplex Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, COMET

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 45M4F9

Format

BSA Free
View all 5'-Nucleotidase/CD73 Primary Antibodies »

Product Specifications

Immunogen

A portion of amino acids 169-324 of human CD73 were used as the immunogen for the antibody.

Specificity

This product can detect Isoform 1 and Isoform of Human CD73.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for 5'-Nucleotidase/CD73 Antibody (45M4F9) - BSA Free

5'-Nucleotidase/CD73 Antibody (45M4F9) - BSA Free

Immersion fixed paraffin embedded sections of Human Lung cancer tissue

CD73 was detected in immersion fixed paraffin-embedded sections of human lung cancer using Mouse Anti-Human CD73 Monoclonal Antibody (Novus Catalog # NBP2-25237) at 1:200 at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cell membrane. Protocol available in COMET™ Panel Builder.
5'-Nucleotidase/CD73 Antibody (45M4F9) - BSA Free

Detection of CD73 in Human Cervical Cancer via Multiplex Immunofluorescence staining on COMET™

CD73 was detected in immersion fixed paraffin-embedded sections of human cervical cancer using Mouse Anti-Human CD73 Monoclonal Antibody (Novus Catalog # NBP2-25237) at 1:200 at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cell membrane. Protocol available in COMET™ Panel Builder.
5'-Nucleotidase/CD73 Antibody (45M4F9) - BSA Free

Detection of CD73 in Human Tonsil via Multiplex Immunofluorescence staining on COMET™

CD73 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human CD73 Monoclonal Antibody (Novus Catalog # NBP2-25237 ) at 1:200 at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cell membrane. Protocol available in COMET™ Panel Builder.
Western Blot: 5'-Nucleotidase/CD73 Antibody (45M4F9)BSA Free [NBP2-25237]

Western Blot: 5'-Nucleotidase/CD73 Antibody (45M4F9)BSA Free [NBP2-25237]

Western Blot: 5'-Nucleotidase/CD73 Antibody (45M4F9) [NBP2-25237] - Analysis of CD73 in a partial recombinant protein using CD73 antibody at 1 ug/ml. Goat anti-mouse IgG HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody (45M4F9) - BSA Free [NBP2-25237]

Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody (45M4F9) - BSA Free [NBP2-25237]

Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody (45M4F9) [NBP2-25237] - Formalin-fixed, paraffin-embedded human breast stained with CD73 antibody (20 ug/ml), peroxidase-conjugate and DAB chromogen.

Applications for 5'-Nucleotidase/CD73 Antibody (45M4F9) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

5 ug/ml

Multiplex Immunofluorescence

1:200

Western Blot

2-5 ug/ml

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: 5'-Nucleotidase/CD73

CD73 is a membrane-bound enzyme having a key role in extracellular ATP metabolism producing adenosine by dephosphorylating AMP. It is highly expressed on endothelial cells but a moderate expression is also observed on a subpopulation of lymphocytes, especially regulatory T cells. Although CD73 on the endothelial cells and lymphocytes are structurally similar, they are differently regulated. Triggering of lymphocyte CD73 with antibodies results in cleavage of CD73 and activation of LFA-1, leading to increased adhesion of lymphocytes to endothelium (Algars A et al., 2011). CD73 plays an important role in carcinogenesis, as adenosine promotes tumor progression and CD73-expressing cancer cell lines are more aggressive (Supernat A et al., 2012). Extracellular adenosine is a potent immunosuppressor that accumulates during tumor growth. Anti-CD73 monoclonal antibody therapy significantly delays primary 4T1.2 and E0771 tumor growth in immune-competent mice and significantly inhibits the development of spontaneous 4T1.2 lung metastases. Antibody therapy is essentially dependent on the induction of adaptive anti-tumor immune responses (Stagg J et al., 2010).

Alternate Names

5-NT, CD73, E5NT, eNT, NT5E, NTE

Gene Symbol

NT5E

UniProt

NP_001191742 (Human)

Additional 5'-Nucleotidase/CD73 Products

Product Documents for 5'-Nucleotidase/CD73 Antibody (45M4F9) - BSA Free

Certificate of Analysis

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Product Specific Notices for 5'-Nucleotidase/CD73 Antibody (45M4F9) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for 5'-Nucleotidase/CD73 Antibody (45M4F9) - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for 5'-Nucleotidase/CD73 Antibody (45M4F9) - BSA Free

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  • Q: We are interested in CD73 antibodies that could react with dog, please let me know which products in your catalog you would recommend.

    A:

    We do not currently carry any CD73 antibodies that have been validated for use in dog samples. However, if you are interested in testing any of our CD73 antibodies with dog samples you would be eligible for our Innovators Reward Program, in which you can get a discount voucher for the purchase price of the product. Please contact us at innovators@novusbio.com with any questions regarding this program.

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