6 Phosphofructo 2 Kinase Antibody (3F3) - Azide and BSA Free

Novus Biologicals | Catalog # H00005209-M08

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Western Blot, ELISA, Immunoprecipitation

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3F3

Format

Azide and BSA Free
View all PFKFB3 Primary Antibodies »

Product Specifications

Immunogen

PFKFB3 (NP_004557, 412 a.a. ~ 520 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. CPLHTVLKLTPVAYGCRVESIYLNVESVCTHRERSEDAKKGPNPLMRRNSVTPLASPEPTKKPRINSFEEHVASTSAALPSCLPPEVPTQLPGQNMKGSRSSADSSRKH

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 22421967). Mouse reactivity reported in scientific literature (PMID: 24169697).

Specificity

PFKFB3 - 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (3F3)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for 6 Phosphofructo 2 Kinase Antibody (3F3) - Azide and BSA Free

Western Blot: 6 Phosphofructo 2 Kinase Antibody (3F3) [H00005209-M08]

Western Blot: 6 Phosphofructo 2 Kinase Antibody (3F3) [H00005209-M08]

Western Blot: 6 Phosphofructo 2 Kinase Antibody (3F3) [H00005209-M08] - Analysis of PFKFB3 expression in transfected 293T cell line by PFKFB3 monoclonal antibody (M08), clone 3F3. Lane 1: PFKFB3 transfected lysatE (59.6 KDa). Lane 2: Non-transfected lysate.
Western Blot: 6 Phosphofructo 2 Kinase Antibody (3F3) [H00005209-M08]

Western Blot: 6 Phosphofructo 2 Kinase Antibody (3F3) [H00005209-M08]

Western Blot: 6 Phosphofructo 2 Kinase Antibody (3F3) [H00005209-M08] - Analysis of PFKFB3 transfected lysate using anti-PFKFB3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PFKFB3 rabbit polyclonal antibody.
ELISA: 6 Phosphofructo 2 Kinase Antibody (3F3) [H00005209-M08] - Detection limit for recombinant GST tagged PFKFB3 is approximately 3ng/ml as a capture antibody.

Applications for 6 Phosphofructo 2 Kinase Antibody (3F3) - Azide and BSA Free

Application
Recommended Usage

Immunoprecipitation

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Reviewed Applications

Read 1 review rated 4 using H00005209-M08 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PFKFB3

Synthesis and degradation of fructose 2,6-bisphosphate

Long Name

6-Phosphofructo-2-Kinase/ Fructose-2,6-Bisphosphatase

Alternate Names

6 Phosphofructo 2 Kinase, IPFK2, PFK/FBPase 3, PFK2

Entrez Gene IDs

5209 (Human)

Gene Symbol

PFKFB3

UniProt

NP_004557 (Human)

Additional PFKFB3 Products

Product Documents for 6 Phosphofructo 2 Kinase Antibody (3F3) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for 6 Phosphofructo 2 Kinase Antibody (3F3) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for 6 Phosphofructo 2 Kinase Antibody (3F3) - Azide and BSA Free

Customer Reviews for 6 Phosphofructo 2 Kinase Antibody (3F3) - Azide and BSA Free (1)

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Customer Images

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  • Name: Amy Clem
    Application: Western Blot
    Sample Tested: A549 Nuclear and cytoplasmic, Sample Amount: 30ug
    Species: Human
    Verified Customer | Posted 12/08/2010
    6 Phosphofructo 2 Kinase Antibody (3F3) - Azide and BSA Free H00005209-M08

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