ABAT Antibody (10V1K9)

Novus Biologicals | Catalog # NBP3-16767

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 10V1K9 expressed in HEK293
View all ABAT Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ABAT (P80404). MASMLLAQRLACSFQHSYRLLVPGSRHISQAAAKVDVEFDYDGPLMKTEVPGPRSQELMKQLNIIQNAEAVHFFCNYEESRGNYLVDVDGNRMLDLYSQI

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ABAT Antibody (10V1K9)

Western Blot: ABAT Antibody (10V1K9) [NBP3-16767]

Western Blot: ABAT Antibody (10V1K9) [NBP3-16767]

Western Blot: ABAT Antibody (10V1K9) [NBP3-16767] - Western blot analysis of extracts of various cell lines, using GABA transaminase (ABAT) Rabbit mAb (NBP3-16767) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry-Paraffin: ABAT Antibody (10V1K9) [NBP3-16767]

Immunohistochemistry-Paraffin: ABAT Antibody (10V1K9) [NBP3-16767]

Immunohistochemistry-Paraffin: ABAT Antibody (10V1K9) [NBP3-16767] - Immunohistochemistry of paraffin-embedded mouse spinal cord using GABA transaminase (ABAT) Rabbit mAb (NBP3-16767) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: ABAT Antibody (10V1K9) [NBP3-16767]

Immunohistochemistry-Paraffin: ABAT Antibody (10V1K9) [NBP3-16767]

Immunohistochemistry-Paraffin: ABAT Antibody (10V1K9) [NBP3-16767] - Immunohistochemistry of paraffin-embedded rat brain using GABA transaminase (ABAT) Rabbit mAb (NBP3-16767) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: ABAT Antibody (10V1K9) [NBP3-16767]

Immunohistochemistry-Paraffin: ABAT Antibody (10V1K9) [NBP3-16767]

Immunohistochemistry-Paraffin: ABAT Antibody (10V1K9) [NBP3-16767] - Immunohistochemistry of paraffin-embedded human liver cancer using GABA transaminase (ABAT) Rabbit mAb (NBP3-16767) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ABAT Antibody (10V1K9)

Immunohistochemistry: ABAT Antibody (10V1K9) [NBP3-16767] -

Immunohistochemistry: ABAT Antibody (10V1K9) [NBP3-16767] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using GABA transaminase Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABAT Antibody (10V1K9)

Immunohistochemistry: ABAT Antibody (10V1K9) [NBP3-16767] -

Immunohistochemistry: ABAT Antibody (10V1K9) [NBP3-16767] - Immunohistochemistry analysis of paraffin-embedded Human liver tissue using GABA transaminase Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Applications for ABAT Antibody (10V1K9)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ABAT

4-aminobutyrate aminotransferase (ABAT) is responsible for catabolism of gamma-aminobutyric acid (GABA), an important, mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. The active enzyme is a homodimer of 50-kD subunits complexed to pyridoxal-5-phosphate. The protein sequence is over 95% similar to the pig protein. GABA is estimated to be present in nearly one-third of human synapses. ABAT in liver and brain is controlled by 2 codominant alleles with a frequency in a Caucasian population of 0.56 and 0.44. The ABAT deficiency phenotype includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found for this gene. [provided by RefSeq]

Long Name

4-aminobutyrate aminotransferase, mitochondrial

Alternate Names

EC 2.6.1.19, EC 2.6.1.22, GABA transaminase, GABA-AT, GABA-T, GABAT, L-AIBAT

Gene Symbol

ABAT

Additional ABAT Products

Product Documents for ABAT Antibody (10V1K9)

Certificate of Analysis

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Product Specific Notices for ABAT Antibody (10V1K9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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