ABCA7 Antibody (7A1-144) - BSA Free

Novus Biologicals | Catalog # NB400-166

Novus Biologicals
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Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2a Kappa Clone # 7A1-144

Format

BSA Free
View all ABCA7 Primary Antibodies »

Product Specifications

Immunogen

Mouse ABCA7 stably-transfected HeLa cells. [UniProt# Q91V24]

Localization

Cell Membrane and Cytoplasmic

Clonality

Monoclonal

Host

Rat

Isotype

IgG2a Kappa

Theoretical MW

240 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ABCA7 Antibody (7A1-144) - BSA Free

Western Blot: ABCA7 Antibody (7A1-144)Unpurified [NB400-166]

Western Blot: ABCA7 Antibody (7A1-144)Unpurified [NB400-166]

Western Blot: ABCA7 Antibody (7A1-144) - Unpurified [NB400-166] - Detection of ABCA7 using NB 400-166 at various dilutions in ABCA7 transfected HeLa cell lysates.
Flow Cytometry: ABCA7 Antibody (7A1-144) - Unpurified [NB400-166]

Flow Cytometry: ABCA7 Antibody (7A1-144) - Unpurified [NB400-166]

Flow Cytometry: ABCA7 Antibody (7A1-144) - Unpurified [NB400-166] - Analysis of 6C (red) (negative control HeLa cells) and A7(blue) (ABCA7 expressing HeLa cells) using NB 400-166 at a dilution of 1:3,000.

Applications for ABCA7 Antibody (7A1-144) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10000-1:60000

Immunohistochemistry-Paraffin

1:100-1:250

Western Blot

1:2000-1:8000
Application Notes
This ABCA7 antibody is useful for Flow Cytometry and Western Blot, where a band is observed at ~240 kDa. This ABCA7 antibody is not reactive in ELISA. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ABCA7

ABCA7 (ATP-binding cassette sub-family A member 7) belongs to "A" subfamily of ABC transporters that predominantly transport lipids and other lipophilic molecules across membranes. ABCA7 shows higher expression in brain, lung, adrenal gland and spleen as well as hematopoietic tissues. It localizes to cell membrane ruffles/phagocytic cups of activated macrophages; to cytoplasm (Golgi and endosomes) of resting macrophages, and to the apical brush border of cells in the proximal tubules of kidney. It is also detected in both the plasma membrane and in intracellular compartments in myelo-lymphatic tissues (peripheral leukocytes, thymus, spleen, and bone marrow). ABCA7 facilitates the transport of phospholipids and, to a lesser extent, cholesterol, across cell membranes to extracellular apoAI and apoE discoidal lipoprotein complexes. Moreover, it implicates in macrophages mediated phagocytosis of apoptotic cells, and binds APOA1 for involving in apolipoprotein-mediated phospholipid efflux from cells. ABCA7 also mediate cholesterol efflux, and can regulate the cellular ceramide homeostasis during keratinocytes differentiation.

Alternate Names

ABCA-SSN, ABCXATP-binding cassette sub-family A member 7, ATP-binding cassette, sub-family A (ABC1), member 7, Autoantigen SS-N, EC 2.2.1.1, EC 3.6.3, EC 3.6.3.41, FLJ40025, Macrophage ABC transporter

Gene Symbol

ABCA7

Additional ABCA7 Products

Product Documents for ABCA7 Antibody (7A1-144) - BSA Free

Certificate of Analysis

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Product Specific Notices for ABCA7 Antibody (7A1-144) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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