ABCA7 Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-14250
![Immunocytochemistry/ Immunofluorescence: ABCA7 Antibody [NBP2-14250]](https://resources.rndsystems.com/images/products/ABCA7-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-14250-img0009.jpg "Immunocytochemistry/ Immunofluorescence: ABCA7 Antibody [NBP2-14250]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
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Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to the amino acids: VNRTFEELTLLRDVREVWEMLGPRIFTFMNDSSNVAMLQRLLQMQDEGRRQPRPGGRDHMEALRSFLDPGSGGYSWQDAHADVGHL
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for ABCA7 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence: ABCA7 Antibody [NBP2-14250]
Immunocytochemistry/Immunofluorescence: ABCA7 Antibody [NBP2-14250] - Staining of human cell line A-431 shows localization to plasma membrane, the Golgi apparatus & cell junctions. Antibody staining is shown in green.![ABCA7 Antibody - BSA Free Immunohistochemistry-Paraffin: ABCA7 Antibody [NBP2-14250]](https://resources.rndsystems.com/images/products/nbp2-14250_-immunohistochemistry-paraffin-639173123157814988.jpg "Immunohistochemistry-Paraffin: ABCA7 Antibody [NBP2-14250]")
Immunohistochemistry-Paraffin: ABCA7 Antibody [NBP2-14250]
Staining of human skeletal muscle shows no positivity in myocytes as expected.![ABCA7 Antibody - BSA Free Immunohistochemistry-Paraffin: ABCA7 Antibody [NBP2-14250]](https://resources.rndsystems.com/images/products/nbp2-14250_-immunohistochemistry-paraffin-639173123329195317.jpg "Immunohistochemistry-Paraffin: ABCA7 Antibody [NBP2-14250]")
Immunohistochemistry-Paraffin: ABCA7 Antibody [NBP2-14250]
Staining of human spleen shows strong cytoplasmic positivity in a subset of cells in red pulp.![ABCA7 Antibody - BSA Free Immunohistochemistry-Paraffin: ABCA7 Antibody [NBP2-14250]](https://resources.rndsystems.com/images/products/nbp2-14250_-immunohistochemistry-paraffin-639173123686936179.jpg "Immunohistochemistry-Paraffin: ABCA7 Antibody [NBP2-14250]")
![ABCA7 Antibody - BSA Free Immunohistochemistry-Paraffin: ABCA7 Antibody [NBP2-14250]](https://resources.rndsystems.com/images/products/nbp2-14250_-immunohistochemistry-paraffin-639173124971320445.jpg "Immunohistochemistry-Paraffin: ABCA7 Antibody [NBP2-14250]")
Immunohistochemistry-Paraffin: ABCA7 Antibody [NBP2-14250]
Staining of human lymph node shows moderate positivity in lymphoid cells.![ABCA7 Antibody - BSA Free Immunohistochemistry-Paraffin: ABCA7 Antibody [NBP2-14250]](https://resources.rndsystems.com/images/products/nbp2-14250_-immunohistochemistry-paraffin-639173132361110893.jpg "Immunohistochemistry-Paraffin: ABCA7 Antibody [NBP2-14250]")
Immunohistochemistry-Paraffin: ABCA7 Antibody [NBP2-14250]
Staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.Applications for ABCA7 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry-Paraffin
1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, Fixation/Permeabilization: PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ABCA7
ABCA-7 is highly homologous to ABCA1, and also mediates cellular cholesterol and phospholipid release by apolipoproteins. The ABCA7 gene is regulated by sterol in the opposite direction to ABCA1 through SRE/SREBP2. The expression of ABCA7 by this regulation is associated with phagocytic activity. As well, it binds APOA1 and may function in apolipoprotein-mediated phospholipid efflux from cells. It also may mediate cholesterol efflux.
ABCA7 antibodies are useful for studies of cholesterol formation and lipid processing mechanisms.
Alternate Names
ABCA-SSN, ABCXATP-binding cassette sub-family A member 7, ATP-binding cassette, sub-family A (ABC1), member 7, Autoantigen SS-N, EC 2.2.1.1, EC 3.6.3, EC 3.6.3.41, FLJ40025, Macrophage ABC transporter
Entrez Gene IDs
10347 (Human)
Gene Symbol
ABCA7
UniProt
Additional ABCA7 Products
Product Documents for ABCA7 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for ABCA7 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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