Q: I would like to use this product for IHC on paraffin. Which kind of antigen retrieval is the best for this product?

A: In our testing of NBP1-18886 in IHC-P, we used the Citrate Buffer pH 6.0 antigen retrieval method.protocol

ABCB9 Antibody

Novus Biologicals | Catalog # NBP1-18886

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

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Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 475 and 525 of human ATP-binding cassette, sub-family B (MDR/TAP), member 9 using the numbering given in entry NP_062571.1 (GeneID 23457).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ABCB9 Antibody

Western Blot: ABCB9 Antibody [NBP1-18886]

Western Blot: ABCB9 Antibody [NBP1-18886] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20%of IP loaded), HEK293T (T; 50 ug) and mouse NIH 3T3 (M; 50ug) cells. Antibodies: Affinity purified rabbit anti-ABCB9 antibody used for WB at 0.04 ug/ml (A) and 1ug/ml (B) and used for IP at 3 ug/mg lysate. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).

Immunohistochemistry-Paraffin: ABCB9 Antibody [NBP1-18886]

Immunohistochemistry-Paraffin: ABCB9 Antibody [NBP1-18886] - Human prostate carcinoma. Antibody used at a dilution of 1:200 (1ug/ml).

Applications for ABCB9 Antibody

Application

Recommended Usage

Immunohistochemistry

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Immunoprecipitation

2-5 ug/mg

Western Blot

1:2000-1:10000

Application Notes

Epitope retrieval with citrate buffer pH6.0 or Tris-EDTA pH9.0 is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ABCB9

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance as well as antigen presentation. The function of this half-transporter has not yet been determined; however, this protein may play a role in lysosomes. Alternative splicing of this gene results in distinct isoforms which are likely to have different substrate specifications. [provided by RefSeq]

Alternate Names

ABC transporter 9 protein, ATP-binding cassette sub-family B member 9, ATP-binding cassette transporter 9, ATP-binding cassette, sub-family B (MDR/TAP), member 9, EC 3.6.3, EC 3.6.3.44, EST122234, hABCB9, KIAA1520, TAPL, TAP-like protein

Entrez Gene IDs

23457 (Human)

Gene Symbol

ABCB9

UniProt

NP_062571.1 (Human)

Additional ABCB9 Products

Product Documents for ABCB9 Antibody

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Certificate of Analysis

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Product Specific Notices for ABCB9 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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