ABHD4 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-15550

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
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Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 223-342 of human ABHD4 (NP_071343.2). PDFKRKFADFFEDDTISEYIYHCNAQNPSGETAFKAMMESFGWARRPMLERIHLIRKDVPITMIYGSDTWIDTSTGKKVKMQRPDSYVRDMEIKGASHHVYADQPHIFNAVVEEICDSVD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit ABHD4 Antibody - Azide and BSA Free (NBP3-15550) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for ABHD4 Antibody - Azide and BSA Free

Western Blot: ABHD4 AntibodyAzide and BSA Free [NBP3-15550]

Western Blot: ABHD4 AntibodyAzide and BSA Free [NBP3-15550]

Western Blot: ABHD4 Antibody [NBP3-15550] - Western blot analysis of extracts of Rat testis, using ABHD4 Rabbit pAb (NBP3-15550) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunocytochemistry/ Immunofluorescence: ABHD4 Antibody - Azide and BSA Free [NBP3-15550]

Immunocytochemistry/ Immunofluorescence: ABHD4 Antibody - Azide and BSA Free [NBP3-15550]

Immunocytochemistry/Immunofluorescence: ABHD4 Antibody [NBP3-15550] - Immunofluorescence analysis of L929 cells using ABHD4 antibody (NBP3-15550) at dilution of 1:100. Blue: DAPI for nuclear staining.

Applications for ABHD4 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ABHD4

Lysophospholipase selective for N-acyl phosphatidylethanolamine (NAPE). Contributes to the biosynthesis ofN-acyl ethanolamines, including the endocannabinoid anandamide by hydrolyzing the sn-1 and sn-2 acyl chains fromN-acyl phosphatidylethanolamine (NAPE) generating glycerophospho-N-acyl ethanolamine (GP-NAE), an intermediate forN-acyl ethanolamine biosynthesis. Hydrolyzes substrates bearing saturated, monounsaturated, polyunsaturated N-acylchains. Shows no significant activity towards other lysophospholipids, including lysophosphatidylcholine,lysophosphatidylethanolamine and lysophosphatidylserine

Alternate Names

ABH4, abhydrolase domain containing 4, abhydrolase domain-containing protein 4, Alpha/beta-hydrolase 4, EC 3.1.1.-, FLJ12816, Lyso-N-acylphosphatidylethanolamine lipase

Gene Symbol

ABHD4

Additional ABHD4 Products

Product Documents for ABHD4 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for ABHD4 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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Protocols

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FAQs

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