ACAT1 Antibody

Novus Biologicals | Catalog # NBP1-51994

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all ACAT1 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-KRVDFSKVPKLKT corresponding to internal region according to NP_000010.1.

Epitope

C-KRVDFSKVPKLKT

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

45.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ACAT1 Antibody

Western Blot: ACAT1 Antibody [NBP1-51994]

Western Blot: ACAT1 Antibody [NBP1-51994]

Western Blot: ACAT1 Antibody [NBP1-51994] - Analysis of Mouse (A) and Rat (B) kidney lysates, and Mouse (C) and Rat (D) Liver lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: ACAT1 Antibody [NBP1-51994]

Western Blot: ACAT1 Antibody [NBP1-51994]

Western Blot: ACAT1 Antibody [NBP1-51994] - (0.01ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for ACAT1 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:8000

Western Blot

0.01 - 0.03 ug/ml
Application Notes
IF: An anonymous customer has reported particulate cytoplasm staining in HeLa WB: Approx. 45 kDa band observed in human liver lysates and in rodent kidney and liver lysates (calculated MW of 45.2 kDa band according to NP_000010.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ACAT1

ACAT-1 is a ~50 kDa protein that catalyzes the formation of intracellular cholesterol esters in various tissues, such as adult human liver, adrenal glands, macrophages, and kidneys.

Long Name

Acetyl-CoA Acetyltransferase 1

Alternate Names

THIL

Entrez Gene IDs

38 (Human)

Gene Symbol

ACAT1

UniProt

NP_000010.1 (Human)

Additional ACAT1 Products

Product Documents for ACAT1 Antibody

Certificate of Analysis

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Download SDS

Product Specific Notices for ACAT1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Bioactive Lipids and Receptors

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