ACAT2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-54616

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ACAT2 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptides corresponding to ACAT2 (acetyl-Coenzyme A acetyltransferase 2 (acetoacetyl Coenzyme A thiolase)) The peptide sequence was selected from the middle region of ACAT2. Peptide sequence SREDQDKVAVLSQNRTENAQKAGHFDKEIVPVLVSTRKGLIEVKTDEFPR The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for ACAT2 Antibody - BSA Free

Western Blot: ACAT2 Antibody [NBP1-54616]

Western Blot: ACAT2 Antibody [NBP1-54616]

Western Blot: ACAT2 Antibody [NBP1-54616] - Titration: 1.0ug/ml Positive Control: K562 cell lysate.
Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-54616]

Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-54616]

Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-54616] - Human Liver Tissue, antibody concentration 4-8ug/ml. Cells with positive label: Hepatocytes (indicated with arrows) 400X magnification.

Applications for ACAT2 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1.0 ug/ml

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 2% Sucrose

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ACAT2

Acetyl-Coenzyme A acetyltransferase 2 is an enzyme involved in lipid metabolism. Reported patients with ACAT2 deficiency have shown severe mental retardation and hypotonus. The ACAT2 gene shows complementary overlapping with the 3-prime region of the TCP1 gene in both mouse and human. These genes are encoded on opposite strands of DNA, as well as in opposite transcriptional orientation.

Long Name

Acetyl-CoA acetyltransferase, cytosolic

Alternate Names

ACTL, EC 2.3.1.9

Gene Symbol

ACAT2

UniProt

Q9BWD1 (Human)

Additional ACAT2 Products

Product Documents for ACAT2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ACAT2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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