ACE/CD143 Antibody (JM59-32)

Novus Biologicals | Catalog # NBP2-67111

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JM59-32 expressed in HEK293
View all ACE/CD143 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human ACE/CD143 aa 1,257-1,306 / 1,306. (SwissProt: P12821 Human; SwissProt: P09470 Mouse)

Localization

Secreted. Cell membrane. Cytoplasm.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ACE/CD143 Antibody (JM59-32)

Western Blot: ACE/CD143 Antibody (JM59-32) [NBP2-67111]

Western Blot: ACE/CD143 Antibody (JM59-32) [NBP2-67111]

Western Blot: ACE/CD143 Antibody (JM59-32) [NBP2-67111] - Analysis of ACE on human lung (1) and mouse kidney (2) tissue lysate using anti-ACE antibody at 1/1,000 dilution.
Immunohistochemistry-Paraffin: ACE/CD143 Antibody (JM59-32) [NBP2-67111]

Immunohistochemistry-Paraffin: ACE/CD143 Antibody (JM59-32) [NBP2-67111]

Immunohistochemistry-Paraffin: ACE/CD143 Antibody (JM59-32) [NBP2-67111] - Analysis of paraffin-embedded mouse testis tissue using anti-ACE antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: ACE/CD143 Antibody (JM59-32) [NBP2-67111]

Immunohistochemistry-Paraffin: ACE/CD143 Antibody (JM59-32) [NBP2-67111]

Immunohistochemistry-Paraffin: ACE/CD143 Antibody (JM59-32) [NBP2-67111] - Analysis of paraffin-embedded human kidney tissue using anti-ACE antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: ACE/CD143 Antibody (JM59-32) [NBP2-67111]

Immunohistochemistry-Paraffin: ACE/CD143 Antibody (JM59-32) [NBP2-67111]

Immunohistochemistry-Paraffin: ACE/CD143 Antibody (JM59-32) [NBP2-67111] - Analysis of paraffin-embedded mouse kidney tissue using anti-ACE antibody. Counter stained with hematoxylin.
ACE/CD143 Antibody (JM59-32) Western Blot: ACE/CD143 Antibody (JM59-32) [NBP2-67111]

Western Blot: ACE/CD143 Antibody (JM59-32) [NBP2-67111]

Western blot analysis of rat kidney tissue lysates with NBP2-67111 at 1/1,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 150 kDa Observed band size: 170 kDa Exposure time: 1 minutes 150 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-67111 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Applications for ACE/CD143 Antibody (JM59-32)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunohistochemistry-Paraffin

1:100-1:500

Western Blot

1:500-1:2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ACE/CD143

ACE converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilatator. ACE exists in two forms, a 170KD somatic form and a 90KD germinal form. The somatic form is expressed by endothelial cells (especially those of lung capillaries and arterioles), epithelial cells (especially in proximal renal tubules and small intestine), by some neuronal cells and variably by some macrophages and T lymphocytes. The germinal form is expressed by spermatozoa.

Long Name

Angiotensin I Converting Enzyme

Alternate Names

CD143, DCP1, Kininase II

Gene Symbol

ACE

Additional ACE/CD143 Products

Product Documents for ACE/CD143 Antibody (JM59-32)

Certificate of Analysis

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Product Specific Notices for ACE/CD143 Antibody (JM59-32)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ACE/CD143 Antibody (JM59-32)

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Protocols

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