ACF Antibody - Azide and BSA Free

Novus Biologicals | Catalog # H00029974-B01P

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Mouse IgG

Format

Azide and BSA Free
View all ACF Primary Antibodies »

Product Specifications

Immunogen

A1CF (NP_620311.1, 1 a.a. - 594 a.a.) full-length human protein. MEAVCLGTCPEPEASMSTAIPGLKKGNNALQSIILQTLLEKENGQRKYGGPPPGWDAAPPERGCEIFIGKLPRDLFEDELIPLCEKIGKIYEMRMMMDFNGNNRGYAFVTFSNKVEAKNAIKQLNNYEIRNGRLLGVCASVDNCRLFVGGIPKTKKREEILSEMKKVTEGVVDVIVYPSAADKTKNRGFAFVEYESHRAAAMARRKLLPGRIQLWGHGIAVDWAEPEVEVDEDTMSSVKILYVRNLMLSTSEEMIEKEFNNIKPGAVERVKKIRDYAFVHFSNREDAVEAMKALNGKVLDGSPIEVTLAKPVDKDSYVRYTRGTGGRGTMLQGEYTYSLGQVYDPTTTYLGAPVFYAPQTYAAIPSLHFPATKGHLSNRAIIRAPSVRGAAGVRGLGGRGYLAYTGLGRGYQVKGDKREDKLYDILPGMELTPMNPVTLKPQGIKLAPQILEEICQKNNWGQPVYQLHSAIGQDQRQLFLYKITIPALASQNPAIHPFTPPKLSAFVDEAKTYAAEYTLQTLGIPTDGGDGTMATAAAAATAFPGYAVPNATAPVSAAQLKQAVTLGQDLAAYTTYEVYPTFAVTARGDGYGTF

Specificity

A1CF - APOBEC1 complementation factor,

Clonality

Polyclonal

Host

Mouse

Isotype

IgG

Scientific Data Images for ACF Antibody - Azide and BSA Free

Western Blot: ACF Antibody [H00029974-B01P]

Western Blot: ACF Antibody [H00029974-B01P]

Western Blot: ACF Antibody [H00029974-B01P] - Analysis of ACF expression in human placenta.
Immunocytochemistry/ Immunofluorescence: ACF Antibody [H00029974-B01P]

Immunocytochemistry/ Immunofluorescence: ACF Antibody [H00029974-B01P]

Immunocytochemistry/Immunofluorescence: ACF Antibody [H00029974-B01P] - Analysis of purified antibody to A1CF on HeLa cell. (antibody concentration 10 ug/ml)
Western Blot: ACF Antibody [H00029974-B01P]

Western Blot: ACF Antibody [H00029974-B01P]

Western Blot: ACF Antibody [H00029974-B01P] - Analysis of A1CF expression in HeLa.
Western Blot: ACF Antibody [H00029974-B01P]

Western Blot: ACF Antibody [H00029974-B01P]

Western Blot: ACF Antibody [H00029974-B01P] - Analysis of A1CF expression in transfected 293T cell line by A1CF polyclonal antibody. Lane 1: ACF transfected lysate(65.34 KDa). Lane 2: Non-transfected lysate.

Applications for ACF Antibody - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against Recombinant Protein with GST tag on ELISA and Western Blot and also on transfected lysate in western blot. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ACF

Mammalian apolipoprotein B mRNA undergoes site-specific C to U deamination, which is mediated by a multi-component enzyme complex containing a minimal core composed of APOBEC-1 and a complementation factor encoded by this gene. The gene product has three non-identical RNA recognition motifs and belongs to the hnRNP R family of RNA-binding proteins. It has been proposed that this complementation factor functions as an RNA-binding subunit and docks APOBEC-1 to deaminate the upstream cytidine. Studies suggest that the protein may also be involved in other RNA editing or RNA processing events. Alternative splicing occurs at this locus and three full-length transcript variants, encoding three distinct isoforms, have been described. Additional splicing has been observed but the full-length nature of these variants has not been determined. [provided by RefSeq]

Alternate Names

ACF65, ACFASPACF64, apo-B RNA editing protein, APOBEC1 complementation factor, apobec-1 complementation factor (ACF) (ASP), APOBEC-1 stimulating protein, APOBEC1CF, APOBEC1-stimulating protein, MGC163391

Entrez Gene IDs

29974 (Human)

Gene Symbol

A1CF

UniProt

NP_620311.1 (Human)

Additional ACF Products

Product Documents for ACF Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for ACF Antibody - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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