Acidic Calponin Antibody (4C4) - Azide and BSA Free

Novus Biologicals | Catalog # H00001266-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4C4

Format

Azide and BSA Free
View all Acidic Calponin Primary Antibodies »

Product Specifications

Immunogen

CNN3 (NP_001830.1, 1 a.a. ~ 329 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MTHFNKGPSYGLSAEVKNKIASKYDHQAEEDLRNWIEEVTGMSIGPNFQLGLKDGIILCELINKLQPGSVKKVNESSLNWPQLENIGNFIKAIQAYGMKPHDIFEANDLFENGNMTQVQTTLVALAGLAKTKGFHTTIDIGVKYAEKQTRRFDEGKLKAGQSVIGLQMGTNKCASQAGMTAYGTRRHLYDPKMQTDKPFDQTTISLQMGTNKGASQAGMLAPGTRRDIYDQKLTLQPVDNSTISLQMGTNKVASQKGMSVYGLGRQVYDPKYCAAPTEPVIHNGSQGTGTNGSEISDSDYQAEYPDEYHGEYQDDYPRDYQYSDQGIDY

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Acidic Calponin Antibody (4C4) - Azide and BSA Free

Western Blot: Acidic Calponin Antibody (4C4) [H00001266-M01]

Western Blot: Acidic Calponin Antibody (4C4) [H00001266-M01]

Western Blot: Acidic Calponin Antibody (4C4) [H00001266-M01] - Analysis of CNN3 expression in transfected 293T cell line by CNN3 monoclonal antibody (M01), clone 4C4.Lane 1: CNN3 transfected lysate (Predicted MW: 36.4 KDa).Lane 2: Non-transfected lysate.
Western Blot: Acidic Calponin Antibody (4C4) [H00001266-M01]

Western Blot: Acidic Calponin Antibody (4C4) [H00001266-M01]

Western Blot: Acidic Calponin Antibody (4C4) [H00001266-M01] - CNN3 monoclonal antibody (M01), clone 4C4. Analysis of CNN3 expression in HepG2.
Sandwich ELISA: Acidic Calponin Antibody (4C4) [H00001266-M01] - Detection limit for recombinant GST tagged CNN3 is 0.1 ng/ml as a capture antibody.

Applications for Acidic Calponin Antibody (4C4) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
It has been used for ELISA and WB.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Acidic Calponin

Acidic Calponin, or CNN3 for short, consists of a 329 amino acid isoform that is 36 kDa, and is involved in the regulation of smooth muscle contraction. Current research is being conducted on the relationship between acidic calponin and several diseases and disorders, including aortic aneurysm and neuronitis. The protein is not associated with any pathways, but it does interact with the proteins FYN, SLX4, ATG5, LCP1, and EMD.

Alternate Names

calponin 3, acidic, Calponin, acidic isoform, calponin-3, dJ639P13.2.2 (acidic calponin 3)

Gene Symbol

CNN3

UniProt

NP_001830.1 (Human)

Additional Acidic Calponin Products

Product Documents for Acidic Calponin Antibody (4C4) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Acidic Calponin Antibody (4C4) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs

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